Schistosome eggs have a direct role in the induction of basophils capable of a high level of IL-4 production: comparative study of single- and bisexual infection of Schistosoma mansoni in vivo.
Schistosome eggs have a direct role in the induction of basophils capable of a high level of IL-4 production: comparative study of single- and bisexual infection of Schistosoma mansoni in vivo.
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DOI：
10.2149/tmh.2009-24
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发表时间：
2010-01-01
影响因子：
4.5
通讯作者：
Ohta, N.
中科院分区：
文献类型：
--
作者：
Anyan, W. K.;Kumagai, T.;Ohta, N.
本文研究了寄生虫卵在小鼠实验性感染中的免疫生物学作用，特别是嗜碱性白细胞的诱导。在BALB/c小鼠中，单或两性感染曼氏血吸虫后，仅在两性感染中观察到脾肿大和肝脏肉芽肿，并与成熟寄生虫卵的沉积平行。嗜碱性粒细胞反应的动力学比较显示，在感染后第7周，两性感染小鼠的骨髓中的数量显着增加，而不是在单一性别感染中的边际增加。在脾脏中，在嗜碱性粒细胞反应中观察到双峰反应;在感染后第4周出现一个小但可重复的峰，在第8周再次增加，这对应于感染小鼠受影响器官中寄生虫卵的启动和成熟。两性感染小鼠脾细胞产生IL-4的时间过程也相同。为了获得更具体的证据，鸡蛋在嗜碱性粒细胞的诱导作用，我们测试使用静脉注射鸡蛋模型。注射鸡卵可诱导小鼠脾细胞嗜碱性粒细胞增多，从第8天开始伴随脾细胞IL-4产生的上调。在该模型中诱导的嗜碱性粒细胞显示通过流式细胞术证实的高水平的IL-4产生，而在该时间点观察到CD 4 + T细胞的微弱水平的IL-4产生。此外，我们还证实了卵沉积是S.曼氏菌感染可能在启动Th 1/2转换过程中起重要作用。体内曼氏菌感染。
Immunobiological roles of schistosome eggs during murine experimental infection were investigated with special reference to the induction of basophilic leukocytes. After single- or bisexual infection with Schistosoma mansoni in BALB/c mice, splenomegaly and liver granulomas were observed only in bisexual infection in parallel with deposition of mature parasite eggs. Comparison of the kinetics of basophil response revealed a marked increase in number in the bone marrow of mice with bisexual infection at the 7th week post infection as opposed to a marginal increase in single-sex infections. In the spleen, bimodal response was observed in the basophil responses; a small but repeatable peak at the 4th week after infection, increasing again at the 8th week, which corresponded to the initiation and maturation of parasite eggs in the affected organs of infected mice. The same time course was observed for IL-4 production by the splenocytes from mice with bisexual infection. To obtain more concrete evidence of the role of eggs in the induction of basophils, we tested using the intravenous egg injection model. Injection of eggs induced basophilia, and it was accompanied by the up-regulation of IL-4 production in splenocytes from the 8th day. Basophils induced in this model showed a high level of IL-4 production confirmed by flow cytometry, while faint levels of IL-4 production were observed for CD4+ T cells at this time point. In addition, we demonstrate that egg deposition is the trigger of basophil induction and activation in the murine experimental model of S. mansoni infection, which might play an essential role in the initiation of Th1/2 conversion during the course of S. mansoni infection in vivo.