Gut Microbiome of Indonesian Adults Differently Associated with Obesity and type 2 Diabetes under Varied Dietary Habits: A Cross-sectional Study in an Asian Developing City, Yogyakarta
Gut Microbiome of Indonesian Adults Differently Associated with Obesity and type 2 Diabetes under Varied Dietary Habits: A Cross-sectional Study in an Asian Developing City, Yogyakarta
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DOI：
10.21203/rs.3.rs-135452/v1
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发表时间：
2020-12
期刊：
影响因子：
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通讯作者：
Phatthanaphong Therdtatha;Y. Song;Masaru Tanaka;M. Mariyatun;Miisaroh Almunifah;N. Manurung;Siska Indriars
中科院分区：
文献类型：
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作者：
Phatthanaphong Therdtatha;Y. Song;Masaru Tanaka;M. Mariyatun;Miisaroh Almunifah;N. Manurung;Siska Indriars
印度尼西亚是一个发展中国家，由于最近饮食和生活方式的急剧变化，面临着人口肥胖和糖尿病日益严重的全国性问题。为了了解印度尼西亚人的肠道微生物组，饮食和健康之间的关系，我们描述了日惹市75名印度尼西亚成年人的粪便微生物组和代谢组，包括21名肥胖者和25名2型糖尿病（T2D）患者，以及他们的饮食和医疗记录。在主成分分析中，微生物组的变化呈三角形分布，由三个优势细菌属驱动，即拟杆菌属，普雷沃菌属和Romboutsia。Rombella驱动的微生物组以低细菌多样性和高初级胆汁酸为特征，与脂肪驱动的肥胖有关。类杆菌驱动的微生物组，抵消普雷沃氏菌，但与瘤胃球菌科伴随着高碳水化合物饮食增加，显示出与T2D指数呈正相关，但与体重指数呈负相关。值得注意的是，脆弱拟杆菌在T2D患者中增加，粪便结合胆汁酸减少，特别是牛磺熊去氧胆酸（一种具有抗糖尿病活性的法尼醇X受体拮抗剂），而这些特征在二甲双胍给药患者中消失。这些结果表明，在不同的饮食习惯下，印度尼西亚成年人的肠道微生物组状态与肥胖和T2D有不同的相关性。
Indonesia is a developing country facing the national problem of the growing obesity and diabetes in its population due to recent drastic dietary and lifestyle changes. To understand the interface between the gut microbiome, diet, and health of Indonesian people, we characterized fecal microbiomes and metabolomes of 75 Indonesian adults in Yogyakarta City, including 21 obese people and 25 type 2 diabetes (T2D) patients, together with their dietary and medical records. Variations of microbiomes showed a triangular distribution in the principal component analysis, driven by three dominant bacterial genera, namely Bacteroides, Prevotella, and Romboutsia. The Romboutsia-driven microbiome, characterized by low bacterial diversity and high primary bile acids, was associated with fat-driven obesity. The Bacteroides-driven microbiome, which counteracted Prevotella but was associated with Ruminococcaceae concomitantly increased with high-carbohydrate diets, showed positive correlation with T2D indices but negative correlation with body mass index. Notably, Bacteroides fragilis was increased in T2D patients with a decrease of fecal conjugated bile acids, particularly tauroursodeoxycholic acid, a farnesoid X receptor antagonist with anti-diabetic activity, while these features disappeared in patients administered metformin. These results indicate that the gut microbiome status of Indonesian adults is differently associated with obesity and T2D under their varied dietary habits.