Using RNA as Molecular Code for Programming Cellular Function
Using RNA as Molecular Code for Programming Cellular Function
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DOI：
10.1021/acssynbio.5b00297
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发表时间：
2016-08-01
影响因子：
4.7
通讯作者：
Jaramillo, Alfonso
中科院分区：
文献类型：
--
作者：
Kushwaha, Manish;Rostain, William;Jaramillo, Alfonso
RNA参与细胞内广泛的重要分子过程，具有不同的功能：调节、酶和结构。再加上其设计的简便性和可预测性，这些特性可以使RNA成为生物工程师控制细胞机械的有用手柄。通过改变细胞过程中的许多RNA连接，有可能朝着特定的设计目标对细胞进行重新编程。我们认为，RNA可以被视为一种分子编程语言，与基于蛋白质的执行平台一起，可以用来重写细胞功能的广泛方面。在这篇综述中，我们对有助于生物学可编程性的RNA部分、方法和相关计算模型的使用进展进行了分类。我们讨论了RNA部分指令表是如何组合在一起来构建复杂的遗传电路的，并回顾了基于RNA的部件和电路的最近应用。我们探索了RNA工程的未来潜力，并认为RNA的可编程性是坚定地建立一个合理设计的合成生物学时代的重要资源。
RNA is involved in a wide-range of important molecular processes in the cell, serving diverse functions: regulatory, enzymatic, and structural. Together with its ease and predictability of design, these properties can lead RNA to become a useful handle for biological engineers with which to control the cellular machinery. By modifying the many RNA links in cellular processes, it is possible to reprogram cells toward specific design goals. We propose that RNA can be viewed as a molecular programming language that, together with protein-based execution platforms, can be used to rewrite wide ranging aspects of cellular function. In this review, we catalogue developments in the use of RNA parts, methods, and associated computational models that have contributed to the programmability of biology. We discuss how RNA part repertoires have been combined to build complex genetic circuits, and review recent applications of RNA-based parts and circuitry. We explore the future potential of RNA engineering and posit that RNA programmability is an important resource for firmly establishing an era of rationally designed synthetic biology.