Discovery and characterization of smORF-encoded bioactive polypeptides.
Discovery and characterization of smORF-encoded bioactive polypeptides.
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DOI：
10.1038/nchembio.1964
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发表时间：
2015-12
影响因子：
14.8
通讯作者：
Couso JP
中科院分区：
文献类型：
--
作者：
Saghatelian A;Couso JP
对基因组、转录组和蛋白质组的分析揭示了数百至数千个翻译的、但未注释的短开放阅读框架（小ORF或smORF）的存在。smORF及其蛋白质产物smORF编码多肽（SEP）的发现揭示了我们对蛋白质编码基因知识的根本空白。不同的研究已经确定了smORF在代谢、凋亡和发育中的中心作用。这些生物活性SEP的发现强调了这类未开发的生物分子的功能潜力。在这里，我们提供了这个新兴领域的概述，并强调化学生物学的机会，以回答这些新基因的基本问题。这些研究将为基因组的蛋白质编码潜力提供新的见解，并确定在生物学和疾病中发挥作用的功能基因。
Analysis of genomes, transcriptomes, and proteomes reveals the existence of hundreds to thousands of translated, yet non-annotated short open reading frames (small ORFs or smORFs). The discovery of smORFs, and their protein products, smORF-encoded polypeptides (SEPs), reveals a fundamental gap in our knowledge of protein-coding genes. Different studies have identified central roles for smORFs in metabolism, apoptosis, and development. The discovery of these bioactive SEPs emphasizes the functional potential of this unexplored class of biomolecules. Here, we provide an overview of this emerging field and highlight the opportunities for chemical biology to answer fundamental questions about these novel genes. Such studies will provide new insights into the protein-coding potential of genomes and identify functional genes with roles in biology and disease.