Method of intracranial pressure monitoring and cerebrospinal fluid sampling in swine
Method of intracranial pressure monitoring and cerebrospinal fluid sampling in swine
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DOI：
10.1258/002367707779399509
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Fruehauf, Nils R.
中科院分区：
文献类型：
--
作者：
Kaiser, Gernot M.;Fruehauf, Nils R.
人们注意到，脑水肿和脑病经常发生在重病或因创伤、缺血、感染甚至代谢紊乱而死亡的患者中。本研究的目的是建立对猪的颅内压（ICP）和脑脊液（CSF）采样的连续监测，以供进一步研究。在额骨旁正中环切术后，使用脑室引流系统对八头猪进行了ICP监测。采集脑脊液和血清样本以测量葡萄糖和蛋白质的水平。钻孔的操作时间为 21 +/- 8 分钟，直到进行 ICP 监测。手术过程中未出现并发症。 ICP 和 CSF 采样的连续监测很容易执行，并且对任何动物都没有任何副作用。尸检时，没有发现医源性病变，监测导管仍然存在。对于需要ICP监测和脑脊液采样的几种类型的研究，该方法可以成功使用。
Cerebral oedema and cncephalopathy have been noted to occur frequently in patients severely ill or dying after trauma, ischaemia, infections or even metabolic disorders. The objective of the present study was to establish continuous monitoring of the intracranial pressure (ICP) and sampling of cerebrospinal fluid (CSF) for further investigations in swine.ICP monitoring was established in eight pigs by using a ventricular drainage system, implemented after paramedian trepanation of the os frontale. CSF and serum samples were taken for measurement of the levels of glucose and protein.Operating time was 21 +/- 8 min for the trepanation until ICP monitoring was performed. No complications occurred during surgery. Continuous monitoring of ICP and CSF sampling was easy to perform, and without any side-effects in any animal. At autopsy, no iatrogenic lesions were found and monitoring catheters were still in place.For several types of research requiring ICP monitoring and sampling of CSF, this method can be used successfully.