Control of development by altered localization of a transcription factor in B-subtilis
Control of development by altered localization of a transcription factor in B-subtilis
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DOI：
10.1016/s1097-2765(00)80377-9
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发表时间：
1999-11-01
期刊：
影响因子：
16
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Quisel, JD;Lin, DCH;Grossman, AD
以.在枯草芽孢杆菌中，染色体分配蛋白Soj（帕拉）和Spo0J（ParB）调节孢子形成的起始。Soj是孢子形成基因表达的负调节因子，Spo0J拮抗Soj功能。利用Soj与绿色荧光蛋白的融合，我们发现Soj定位在细胞两极附近，并且在进入稳定期时从一极振荡到另一极。在Spo0J不存在的情况下，Soj主要与DNA相关。通过体内交联和免疫沉淀，我们发现Soj与发育调节启动子物理关联，并且这种关联在Spo0J不存在的情况下增加。这些结果表明，Soj开关的定位和功能取决于染色体分配蛋白Spo0J。我们进一步表明，在Soj ATP酶结构域的突变破坏定位和功能，使Soj不敏感的Spo0J调节。
In a. subtilis, the chromosome partitioning proteins Soj (ParA) and Spo0J (ParB) regulate the initiation of sporulation. Soj is a negative regulator of sporulation gene expression, and Spo0J antagonizes Soj function. Using fusions of Soj to green fluorescent protein, we found that Soj localized near the cell poles and upon entry into stationary phase oscillated from pole to pole. In the absence of Spo0J, Soj was associated predominantly with DNA. By in vivo cross-linking and immunoprecipitation, we found that Soj physically associates with developmentally regulated promoters, and this association increased in the absence of Spo0J. These results show that Soj switches localization and function depending on the chromosome partitioning protein Spo0J. We further show that mutations in the Soj ATPase domain disrupt localization and function and render Soj insensitive to regulation by Spo0J.