Exercise-induced expression of monocarboxylate transporter 2 in the cerebellum and its contribution to motor performance.
Exercise-induced expression of monocarboxylate transporter 2 in the cerebellum and its contribution to motor performance.
复制标题
运动诱导的小脑中单羧酸转运蛋白 2 的表达及其对运动性能的贡献。
DOI：
10.1016/j.neulet.2016.09.012
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发表时间：
2016
期刊：
影响因子：
2.5
通讯作者：
Dai Yanagihara.
中科院分区：
文献类型：
--
作者：
Daisuke Hoshino;Susumu Setogawa;Yu Kitaoka;Hiroyuki Masuda;Yuki Tamura;Hideo Hatta;Dai Yanagihara.
单羧酸转运体2（MCT2）是成年脑神经元乳酸转运系统的重要组成部分。小脑中的浦肯野细胞已被证明具有高水平的MCT 2，这表明该蛋白在这些细胞的能量代谢和神经元活动中具有关键功能。然而，目前尚不清楚小脑中通过MCT 2抑制乳酸转运是否会影响运动性能。为了解决这个问题，我们在使用α-氰基-4-羟基肉桂酸（4-CIN）抑制小脑中通过MCT 2的乳酸转运后，检查了小鼠的运动表现。4-将CIN或生理盐水注射到小鼠小脑的蛛网膜下腔中，并在注射之前和之后通过旋转棒测试分析运动性能。4-与注射前相比，注射CIN后1小时，旋转杆试验中的保留时间缩短约80%。注射后2 h或在溶剂对照组小鼠中未观察到任何影响。由于我们观察到MCT 2在运动表现中起着重要作用，我们接下来研究了急性运动对运动前和跑步机跑步2小时后0和5小时采样的小鼠中MCT 2转录和蛋白水平的影响。我们发现运动后5小时小脑中MCT 2 mRNA水平显著增加，但蛋白水平没有增加。我们的研究结果表明，乳酸转运通过MCT2在小脑中可能发挥重要作用的运动性能和运动可以增加MCT2的表达在转录水平上。
Monocarboxylate transporter 2 (MCT2) is an important component of the lactate transport system in neurons of the adult brain. Purkinje cells in the cerebellum have been shown to have high levels of MCT2, suggesting that this protein has a key function in energy metabolism and neuronal activities in these cells. However, it is not known whether inhibition of lactate transport via MCT2 in the cerebellum affects motor performance. To address this question, we examined motor performance in mice following the inhibition of lactate transport via MCT2 in the cerebellum using α-cyano-4-hydroxycinnamate (4-CIN). 4-CIN or saline was injected into the subarachnoidal space of the cerebellum of mice and motor performance was analyzed by a rotarod test both before and after injection. 4-CIN injection reduced retention time in the rotarod test by approximately 80% at 1 h post-injection compared with pre-injection. No effect was observed at 2 h post-injection or in mice treated with the vehicle control. Because we observed that MCT2 plays an important role in motor performance, we next investigated the effects of acute exercise on MCT2 transcription and protein levels in mice sampled pre-exercise and at 0 and 5 h after 2 h of treadmill running. We found a significant increase in MCT2 mRNA levels, but not of protein levels, in the cerebellum at 5 h after exercise. Our results indicate that lactate transport via MCT2 in the cerebellum may play an important role in motor performance and that exercise can increase MCT2 expression at the transcriptional level.