rK39: A cloned antigen of Leishmania chagasi that predicts active visceral leishmaniasis
rK39: A cloned antigen of Leishmania chagasi that predicts active visceral leishmaniasis
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DOI：
10.1093/infdis/173.3.758
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发表时间：
1996-03-01
影响因子：
6.4
通讯作者：
Reed, SG
中科院分区：
文献类型：
--
作者：
Badaro, R;Benson, D;Reed, SG
内脏利什曼病(VL)是由杜氏利什曼原虫复合体成员引起的一种严重且往往是致命的寄生虫病。目前的诊断方法仍然存在问题，目前的方法依赖于临床标准、吸入物中寄生虫的鉴定和血清学。后一种方法使用缺乏特异性的粗抗原制剂。先前描述的针对杜氏利什曼原虫复合体所有成员的克隆抗原rK39在人和犬VL的ELISA诊断中非常有用。本研究证明rK39血清反应性与活动性疾病有关，早期或自愈感染者的血清与利什曼裂解物反应，一般与rK39不反应。这些数据表明rK39在VL的血清学诊断和作为活动期疾病的指标中的实用性。
The diagnosis of visceral leishmaniasis (VL), a serious and often fatal parasitic disease caused by members of the Leishmania donovani complex, remains problematic, Current methods rely on clinical criteria, parasite identification in aspirate material, and serology, The latter methods use crude antigen preparations lacking in specificity, A previously described cloned antigen, rK39, of Leishmania specific for all members of the L. donovani complex (L. chagasi, L. donovani, L. infantum) was very useful in the serodiagnosis by ELISA of both human and canine VL, The present study demonstrated that rK39 seroreactivity correlated with active disease, The sera from early or self-healing infected subjects reacted with leishmanial lysate and were generally nonreactive with rK39. These data demonstrate the utility of rK39 in the serodiagnosis of VL and as an indicator of active disease.