A simple voltage controlled enzymatic nanoreactor produced in the tip of a nanopipet
A simple voltage controlled enzymatic nanoreactor produced in the tip of a nanopipet
复制标题
DOI：
10.1021/nl049029h
复制
发表时间：
2004-10-01
期刊：
影响因子：
10.8
通讯作者：
Klenerman, D
中科院分区：
文献类型：
--
作者：
Bruckbauer, A;Zhou, DJ;Klenerman, D
我们报告了碱性磷酸酶（AP）限制在纳米吸量管的尖端，体积约为100阿升的荧光素二磷酸（FDP）的酶裂解。可逆地吸附在移液管尖端中的酶的量显示与浴中的酶浓度成比例。将施加到移液管的电压增加到1.5 V，通过增加底物流出移液管来线性控制底物周转率。这项工作开辟了高度小型化和灵敏的酶测定与高空间分辨率的可能性。
We report the enzymatic cleavage of fluorescein diphosphate (FDP) by alkaline phosphatase (AP) confined in the tip of a nanopipet with a volume of approximately 100 attoliters. The amount of enzyme that reversibly adsorbs in the pipet tip was shown to be proportional to the enzyme concentration in the bath. Increasing the voltage applied to the pipet, up to 1.5 V, linearly controls the substrate turnover rate by increasing the flow of substrate out of the pipet. This work opens up possibilities of highly miniaturized and sensitive enzyme assays with high spatial resolution.