Acute aflatoxin B1-induced gastro-duodenal and hepatic oxidative damage is preceded by time-dependent hyperlactatemia in rats
Acute aflatoxin B1-induced gastro-duodenal and hepatic oxidative damage is preceded by time-dependent hyperlactatemia in rats
复制标题
DOI：
10.1007/s12550-020-00408-6
复制
发表时间：
2020-09-21
影响因子：
3
通讯作者：
Akinrinmade, Fadeyemi Joseph
中科院分区：
文献类型：
--
作者：
Akinrinde, Akinleye Stephen;Ogunbunmi, Temitope;Akinrinmade, Fadeyemi Joseph
血清乳酸浓度升高已被用来预测急症患者各种疾病状态下的死亡风险或不同化学品中毒的风险。然而，其在预测急性黄曲霉中毒期间疾病进展方面的效用尚未得到研究。本研究旨在评估急性暴露于黄曲霉毒素 B-1 (AFB(1)) 后血乳酸水平的变化，并确定血乳酸水平的变化是否与胃、十二指肠和肝脏的生化和/或形态学病变有任何关系。将21只雄性Wistar大鼠随机分为三组（n = 7只大鼠/组），包括仅接受载体的A组（对照）和分别以2.5和5 mg/kg单次口服剂量的AFB(1)治疗的B组和C组。 AFB(1) 早在给药后 1 小时就产生了显着的（p<0.05）时间和剂量依赖性的血乳酸浓度增加，并在 3 小时和 6 小时观察到进一步增加。高乳酸血症伴随组织氧化变化，包括H(2)O(2)和MDA增加，以及胃十二指肠和肝组织中谷胱甘肽、谷胱甘肽过氧化物酶、超氧化物歧化酶和总硫醇的消耗。氧化变化反映在组织病理学观察到的形态学改变中，较高剂量的 AFB (1) 观察到更严重的病变。然而，使用 AFB(1) 后，促炎细胞因子（TNF-α 和 IL-1β）的血清水平发生了不同程度的改变。总而言之，这项研究的结果表明，高乳酸血症可能有助于预测急性 AFB(1) 暴露引起的组织损伤的严重程度。
Elevated serum lactate concentration has been used to predict the risk of fatality in various disease states in acutely ill patients or poisoning with different chemicals. However, its utility in predicting disease progression during acute aflatoxicosis has not been investigated. This study was designed to evaluate changes in blood lactate levels following acute exposure to aflatoxin B-1(AFB(1)) and to determine whether changes in blood lactate levels bear any relationship with biochemical and/or morphological lesions in the stomach, duodenum, and liver. Twenty-one male Wistar rats were randomly divided into three groups (n= 7 rats /group) including Group A (control) receiving vehicle alone and Groups B and C treated with single oral doses of AFB(1)at 2.5 and 5 mg/kg, respectively. AFB(1)produced significant (p< 0.05) time- and dose-dependent increase in blood lactate concentration as early as 1 h following its administration, with further increases observed at 3 h and 6 h. The hyperlactatemia accompanied tissue oxidative changes including increased H(2)O(2)and MDA, as well as depletion in glutathione, glutathione peroxidase, superoxide dismutase, and total thiols in gastro-duodenal and hepatic tissues. The oxidative changes were reflected in morphological alterations observed at histopathology with more severe lesions observed with the higher dose of AFB(1). Serum levels of pro-inflammatory cytokines (TNF-alpha and IL-1 beta) were, however, differently modified by AFB(1)administration. Taken together, the results from this study gives indication that hyperlactatemia may find utility in predicting the severity of tissue damage induced by acute AFB(1)exposure.