Protein-protein interactions mapped by artificial proteases:: where σ factors bind to RNA polymerase
Protein-protein interactions mapped by artificial proteases:: where σ factors bind to RNA polymerase
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DOI：
10.1016/s0968-0004(00)01652-2
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发表时间：
2000-09-01
影响因子：
13.8
通讯作者：
Meares, CF
中科院分区：
文献类型：
--
作者：
Datwyler, SA;Meares, CF
蛋白质之间的相互作用对于理解很重要，但很难研究。将蛋白质与小型人工蛋白酶结合，使其能够切割与其结合的其他蛋白质。分析靶蛋白上的切割位点可以使人们对每个复合物的结构有新的了解。因子与 RNA 聚合酶上共同区域的结合就是一个例子。
Interactions between proteins are important to understand but difficult to study. Conjugating a protein to a small artificial protease endows it with the ability to cut other proteins where it binds to them. Analysing the sites cut on the target proteins leads to new understanding of the structure of each complex. The binding of a factors to a common region on RNA polymerase provides an example.