Light-induced expression of genes involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis (L.) O. Kuntze)
Light-induced expression of genes involved in phenylpropanoid biosynthetic pathways in callus of tea (Camellia sinensis (L.) O. Kuntze)
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茶愈伤组织中苯丙素生物合成途径相关基因的光诱导表达（Camellia sinensis (L.) O. Kuntze）
DOI：
10.1016/j.scienta.2011.10.017
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发表时间：
2012-01
影响因子：
4.3
通讯作者：
Tao Xia
中科院分区：
文献类型：
--
作者：
YunSheng Wang;LiPing Gao;ZhengRong Wang;YaJun Liu;MeiLian Sun;DongQing Yang;ChaoLing Wei;Yu Shan;Tao Xia
茶树（Camellia sinensis（L.）O. Kuntze）是一种重要的商业作物，其次级代谢产物具有价值。光是调节植物生长发育的重要环境参数，影响植物体内苯丙烷类代谢。为探讨光调控苯丙素代谢的分子机制，建立了茶树愈伤组织光诱导抑制消减杂交（SSH）cDNA文库。本研究共从文库中筛选出265个克隆，进行测序和分析。在文库中检测到9个参与苯丙素类生物合成的不同EST。克隆了一个新的CsDFR基因（CsDFR2），其光激活表达量高于已报道的CsDFR基因（CsDFR1）。通过对关键苯丙素类化合物和代表性基因表达的分析，提示光照可以有效激活苯丙素类化合物的生物合成。与黑暗对照相比，木质素、儿茶素和多胺的含量分别增加了3.46、3.00和1.21倍。在光诱导的SSH文库中筛选到了与CCoAOMT、HCT和CCR相关的木质素合成基因。因此推测木质素可能是茶树愈伤组织中光激活的主要苯丙素类代谢产物。此外，研究还发现，茶树愈伤组织中主要次生代谢产物儿茶素的含量较成熟叶片显著降低，而儿茶素多聚体（PAs）含量较成熟叶片没有降低。聚合反应可能是茶树愈伤组织黄酮代谢的主要途径。本研究建立的SSH文库为进一步了解茶树光诱导次生代谢的机制提供了宝贵的资源。
Tea (Camellia sinensis (L.) O. Kuntze) is a commercially important crop that is valued for its secondary metabolites. Light is an important environmental parameter that regulates plant growth and development and influences the phenylpropanoid metabolism in plants. To investigate the molecular mechanism by which light regulates phenylpropanoid metabolism, we established light-induced suppression subtractive hybridization (SSH) cDNA libraries of tea calli. A total of 265 clones from the library were selected, sequenced, and analyzed in this study. Nine diverse ESTs involved in phenylpropanoid biosynthesis were detected in the library. A new CsDFR gene (CsDFR2), higher increment of the expression activated by light than the previously reported CsDFR gene (CsDFR1), was cloned. The key phenylpropanoid compounds and representative genes expression analysis implied that light could be effective for activation of the biosynthesis of phenylpropanoids. Compared to the darkness control, levels of lignins, catechins, and PAs were increased 3.46, 3.00, and 1.21-fold, in light-induced calli, respectively. And lignin biosynthesis genes, involved in CCoAOMT, HCT and CCR, were identified in the light-induced SSH library. Therefore it was assumed that lignins might be the main phenylpropanoid metabolites activated by light in tea calli. In addition, our researches found that catechins, as the main secondary metabolites, significantly decreased in the tea calli compared to those in tea mature leaves, While PAs (polymer of catechins) in calli did not decrease compared to mature leaves. The data suggest that polymerization reaction might be the main pathway of flavonoid metabolism in tea callus. The SSH library established in this study represents a valuable resource for better understanding the mechanisms of light-induced secondary metabolism in tea plants.
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影响因子：
3.8
作者：
Lo Piero, AR;Puglisi, I;Petrone, G
通讯作者：
Petrone, G
影响因子：
7.4
作者：
D. Xie;Lisa A. Jackson;J. Cooper;D. Ferreira;N. Paiva
通讯作者：
D. Xie;Lisa A. Jackson;J. Cooper;D. Ferreira;N. Paiva
影响因子：
13.8
作者：
Coleman, HD;Ellis, DD;Mansfield, SD
通讯作者：
Mansfield, SD
影响因子：
7.4
作者：
Pang, Yongzhen;Wenger, Jonathan P.;Dixon, Richard A.
通讯作者：
Dixon, Richard A.
影响因子：
4
作者：
F. Nagy;S. Kircher;E. Schäfer
通讯作者：
F. Nagy;S. Kircher;E. Schäfer