Anti-Hepatitis B Virus Activity of Esculetin from Microsorium fortunei In Vitro and In Vivo
Anti-Hepatitis B Virus Activity of Esculetin from Microsorium fortunei In Vitro and In Vivo
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小孢子草七叶亭的体内外抗乙型肝炎病毒活性
DOI：
10.3390/molecules24193475
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发表时间：
2019-10-01
期刊：
影响因子：
4.6
通讯作者：
Liang, Cheng-Qin
中科院分区：
文献类型：
--
作者：
Huang, Si-Xin;Mou, Jun-Fei;Liang, Cheng-Qin
Coumarins are widely present in a variety of plants and have a variety of pharmacological activities. In this study, we isolated a coumarin compound from Microsorium fortunei (Moore) Ching; the compound was identified as esculetin by hydrogen and carbon spectroscopy. Its anti-hepatitis B virus (HBV) activity was investigated in vitro and in vivo. In the human hepatocellular liver carcinoma 2.2.15 cell line (HepG2.2.15) transfected with HBV, esculetin effecting inhibited the expression of the HBV antigens and HBV DNA in vitro. Esculetin inhibited the expression of Hepatitis B virus X (HBx) protein in a dose-dependent manner. In the ducklings infected with duck hepatitis B virus (DHBV), the levels of DHBV DNA, duck hepatitis B surface antigen (DHBsAg), duck hepatitis B e-antigen (DHBeAg), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) decreased significantly after esculetin treatment. Summing up the above, the results suggest that esculetin efficiently inhibits HBV replication both in vitro and in vivo, which provides an opportunity for further development of esculetin as antiviral drug.
