Superparamagnetic iron oxide nanoparticle targeting of adipose tissue-derived stem cells in diabetes-associated erectile dysfunction
Superparamagnetic iron oxide nanoparticle targeting of adipose tissue-derived stem cells in diabetes-associated erectile dysfunction
复制标题
超顺磁性氧化铁纳米颗粒靶向脂肪组织来源的干细胞治疗糖尿病相关的勃起功能障碍
DOI：
10.4103/1008-682x.179532
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发表时间：
2017-07-01
影响因子：
2.9
通讯作者：
Dai, Yu-Tian
中科院分区：
文献类型：
--
作者：
Zhu, Lei-Lei;Zhang, Zheng;Dai, Yu-Tian
勃起功能障碍（艾德）是糖尿病的主要并发症，并且许多患有艾德的糖尿病男性对常见的艾德疗法是难治的。脂肪组织来源的干细胞（ADSC）已被证明可以改善糖尿病动物模型的勃起功能。然而，细胞归巢到受损部位的不足限制了它们的功效。因此，我们探讨了超顺磁性氧化铁纳米颗粒（SPIONs）标记的脂肪干细胞在外加磁场下改善链脲佐菌素诱导的糖尿病大鼠勃起功能的作用。我们发现SPION有效地整合到ADSC中，并且对干细胞特性没有任何负面影响。与单独注射ADSC相比，ADSC的磁靶向有助于阴茎海绵体中的长期细胞保留，并改善糖尿病大鼠的勃起功能。此外，脂肪干细胞的旁分泌效应似乎在功能和结构的恢复中起主要作用。因此，磁场引导的ADSC治疗是治疗糖尿病相关艾德性ED的有效方法。
Erectile dysfunction (ED) is a major complication of diabetes, and many diabetic men with ED are refractory to common ED therapies. Adipose tissue-derived stem cells (ADSCs) have been shown to improve erectile function in diabetic animal models. However, inadequate cell homing to damaged sites has limited their efficacy. Therefore, we explored the effect of ADSCs labeled with superparamagnetic iron oxide nanoparticles (SPIONs) on improving the erectile function of streptozotocin-induced diabetic rats with an external magnetic field. We found that SPIONs effectively incorporated into ADSCs and did not exert any negative effects on stem cell properties. Magnetic targeting of ADSCs contributed to long-term cell retention in the corpus cavernosum and improved the erectile function of diabetic rats compared with ADSC injection alone. In addition, the paracrine effect of ADSCs appeared to play the major role in functional and structural recovery. Accordingly, magnetic field-guided ADSC therapy is an effective approach for diabetes-associated ED therapy.