Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/β-catenin signaling
Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/β-catenin signaling
复制标题
Wnt/β-catenin信号通路缺失下丘脑放射状胶质细胞作为自我更新神经祖细胞的功能
DOI：
10.1242/dev.126813
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发表时间：
2016-01-01
期刊：
影响因子：
4.6
通讯作者：
Dorsky, Richard I.
中科院分区：
文献类型：
--
作者：
Duncan, Robert N.;Xie, Yuanyuan;Dorsky, Richard I.
The vertebrate hypothalamus contains persistent radial glia that have been proposed to function as neural progenitors. In zebrafish, a high level of postembryonic hypothalamic neurogenesis has been observed, but the role of radial glia in generating these new neurons is unclear. We have used inducible Cre-mediated lineage labeling to show that a population of hypothalamic radial glia undergoes self-renewal and generates multiple neuronal subtypes at larval stages. Whereas Wnt/beta-catenin signaling has been demonstrated to promote the expansion of other stem and progenitor cell populations, we find that Wnt/beta-catenin pathway activity inhibits this process in hypothalamic radial glia and is not required for their self-renewal. By contrast, Wnt/beta-catenin signaling is required for the differentiation of a specific subset of radial glial neuronal progeny residing along the ventricular surface. We also show that partial genetic ablation of hypothalamic radial glia or their progeny causes a net increase in their proliferation, which is also independent of Wnt/beta-catenin signaling. Hypothalamic radial glia in the zebrafish larva thus exhibit several key characteristics of a neural stem cell population, and our data support the idea that Wnt pathway function may not be homogeneous in all stem or progenitor cells.
