Phagocytic processing of the macrophage endoparasite, Mycobacterium avium, in comparison to phagosomes which contain Bacillus subtilis or latex beads.
Phagocytic processing of the macrophage endoparasite, Mycobacterium avium, in comparison to phagosomes which contain Bacillus subtilis or latex beads.
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与含有枯草芽孢杆菌或乳胶珠的吞噬体相比，巨噬细胞内寄生虫、鸟分枝杆菌的吞噬过程。
DOI：
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发表时间：
1995
影响因子：
6.6
通讯作者：
L. Thilo
中科院分区：
文献类型：
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作者：
C. Chastellier;Thierry Lang;L. Thilo
在小鼠骨髓源性巨噬细胞中研究了致病性分枝杆菌的吞噬体内生存策略。这些细菌通过以未知方式干扰吞噬体加工而在吞噬体内存活，所述吞噬体加工通常会导致吞噬溶酶体中的吞噬颗粒的消化。在这里，吞噬体加工比较不同的吞噬颗粒：活的鸟分枝杆菌，可降解的枯草芽孢杆菌，或难消化的乳胶珠。我们展示了详细的电子显微镜形态学观察，其特征在于内吞细胞器和吞噬体之间的相互作用的各个阶段。我们测量融合的吞噬体与早期内体或与溶酶体通过使用新的内化内吞内容物（辣根过氧化物酶，HRP）和膜标记物（质膜糖缀合物标记的[3 H]半乳糖通过exglycosylation）。这些观察结果的形态学分析表明，吞噬颗粒的性质影响吞噬体加工：只要颗粒保持未消化，就阻止了吞噬体的成熟，并且它们保持对早期内体的融合;与颗粒消化同时，吞噬体加工朝向将吞噬内容物转移至吞噬溶酶体进行，其显示溶酶体的动力学和组成特征。M.鸟体保留在未成熟的吞噬体中，其与早期内体融合，但不与溶酶体融合。与早期核内体的融合减少，从而表明该内寄生物发挥其作用的阶段。
The intraphagosomal survival strategy of pathogenic mycobacteria was studied in bone marrow-derived mouse macrophages. These bacteria survive inside phagosomes by interfering in an unknown manner with phagosome processing which normally would lead to digestion of the phagocytic particle in phagolysosomes. Here, phagosome processing was compared for different phagocytic particles: live Mycobacterium avium, degradable Bacillus subtilis, or indigestible latex beads. We show detailed electron microscopic morphological observations which characterize various phases of interaction between endocytic organelles and phagosomes. We measured fusion of phagosomes with early endosomes or with lysosomes by using newly internalized endocytic contents (horseradish peroxidase, HRP) and membrane marker (plasma membrane glycoconjugates labeled with [3H]galactose via exoglycosylation). Morphometric analysis of these observations showed that the nature of the phagocytic particle affects phagosome processing: As long as particles remain undigested, maturation of phagosomes is prevented and they remain fusogenic towards early endosomes; concurrent to particle digestion, phagosome processing proceeds towards transfer of phagocytic contents to phagolysosomes which display kinetic and compositional characteristics of lysosomes. As an intact phagocytic particle, M. avium remains in non-matured phagosomes which fuse with early endosomes, but not with lysosomes. Fusion with early endosomes is reduced, thereby indicating the stage where this endoparasite exerts its effect.