Developmental change of GABAA receptor-mediated current in rat hippocampus
Developmental change of GABAA receptor-mediated current in rat hippocampus
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DOI：
10.1016/s0306-4522(99)00574-6
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发表时间：
2000-03
期刊：
影响因子：
3.3
通讯作者：
M. Taketo;T. Yoshioka
中科院分区：
文献类型：
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作者：
M. Taketo;T. Yoshioka
用膜片钳技术观察了大鼠海马CA 3区GABA能抑制性突触后电流的发育性变化。从新生儿（出生后2-4天）和成人（18-38天）的急性海马脑片记录自发和诱发的抑制性突触后电流。新生儿自发抑制性突触后电流的衰减动力学比成人慢。应用500 nM唑吡坦增加了两组中抑制性突触后电流的衰减时间常数，对成人的影响更强。锌（50μM）抑制新生大鼠抑制性突触后电流，但对成年大鼠抑制作用较弱。呋塞米（0.6mM）或地西泮（100 nM）对GABA能抑制性突触后电流的修饰在新生儿组和成人组之间没有引起显著差异。这些结果表明，GABA能抑制性突触后电流在海马CA 3锥体细胞的变化发育，并表明不同的受体亚型的功能表达之间的新生儿和成人。
Developmental change of GABAAergic inhibitory postsynaptic current in rat hippocampal CA3 region was examined using patch-clamp recording method. Spontaneous and evoked inhibitory postsynaptic currents were recorded from acute hippocampal slices of neonates (postnatal days 2–4) and adults (days 18–38). Decay kinetics of the spontaneous inhibitory postsynaptic current was slower in neonates than in adults. Application of 500nM zolpidem increased decay time-constants of the inhibitory postsynaptic currents in both groups with a stronger effect on adults. Zinc (50μM) inhibited the neonatal inhibitory postsynaptic currents but the inhibition was weaker in adults. Modification of the GABAAergic inhibitory postsynaptic currents by furosemide (0.6mM) or diazepam (100nM) did not cause marked differences between the neonate and adult groups. These results demonstrate that GABAAergic inhibitory postsynaptic currents in hippcampal CA3 pyramidal cells change developmentally and indicate that different receptor isoforms are functionally expressed between neonates and adults.