Crystal Structures of the HypCD Complex and the HypCDE Ternary Complex: Transient Intermediate Complexes during [NiFe] Hydrogenase Maturation
Crystal Structures of the HypCD Complex and the HypCDE Ternary Complex: Transient Intermediate Complexes during [NiFe] Hydrogenase Maturation
复制标题
HypCD 复合物和 HypCDE 三元复合物的晶体结构：[NiFe] 氢化酶成熟过程中的瞬时中间复合物
DOI：
10.1016/j.str.2012.09.018
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发表时间：
2012
期刊：
影响因子：
5.7
通讯作者：
and K. Miki
中科院分区：
文献类型：
--
作者：
S. Watanabe;R. Matsumi;H. Atomi;T. Imanaka;and K. Miki
[NiFe]氢化酶成熟代表了一个最具活力和复杂的过程中的组装中心。[NiFe]氢化酶的Fe（CN）2CO部分通过特定成熟蛋白HypC（金属伴侣）、HypD（氧化还原蛋白）和HypE（氰化物合成/供体）之间未知的瞬时相互作用组装。在这里，我们报告的HypC-HypD和HypC-HypD-HypE复合物的结构，提供了一个视图的瞬态相互作用，发生在成熟过程中。HypC通过广泛的疏水相互作用与HypD的保守区结合。HypE和HypCD复合物之间的三元复合物形成涉及HypC和HypD，使得HypE构象有利于氰化物转移。在复合物中，HypC和HypD的保守半胱氨酸形成Fe结合位点。HypE的保守C-末端半胱氨酸可以进入HypD的巯基氧化还原级联。这些结果提供了结构上的见解铁原子氰化HypCDE复合物。
[NiFe] hydrogenase maturation represents one of the most dynamic and sophisticated processes in metallocenter assembly. The Fe(CN)2CO moiety of [NiFe] hydrogenases is assembled via unknown transient interactions among specific maturation proteins HypC (metallochaperone), HypD (redox protein), and HypE (cyanide synthesis/donor). Here, we report the structures of the HypC-HypD and HypC-HypD-HypE complexes, providing a view of the transient interactions that take place during the maturation process. HypC binds to the conserved region of HypD through extensive hydrophobic interactions. The ternary complex formation between HypE and the HypCD complex involves both HypC and HypD, rendering the HypE conformation favorable for cyanide transfer. In the complex, the conserved cysteines of HypC and HypD form an Fe binding site. The conserved C-terminal cysteine of HypE can access the thiol redox cascade of HypD. These results provide structural insights into the Fe atom cyanation in the HypCDE complex.