Achieving Mainstream Nitrogen Removal through Coupling Anammox with Denitratation
Achieving Mainstream Nitrogen Removal through Coupling Anammox with Denitratation
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通过厌氧氨氧化与脱硝耦合实现主流脱氮。
DOI：
10.1021/acs.est.7b01866
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发表时间：
2017-08-01
影响因子：
11.4
通讯作者：
Peng, Yongzhen
中科院分区：
文献类型：
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作者：
Ma, Bin;Qian, Wenting;Peng, Yongzhen
实现主流厌氧氨氧化是能源中性污水处理的关键。本研究提出了一种实现主流厌氧氨氧化的新方法，将厌氧氨氧化与反硝化（硝酸盐还原为亚硝酸盐）而不是亚硝化（铵氧化为亚硝酸盐）耦合。缺氧/好氧（A/O）生物膜系统处理合成生活污水被用来证明这一概念超过400天。在C/N为2.6、TN浓度为60.5mg/L的低C/N比条件下，A/O生物膜系统对总氮（TN）的去除率为80 ± 4%。通过厌氧氨氧化脱氮被发现占70%的双氮生产在缺氧反应器。批式试验证实，缺氧生物膜能以亚硝酸盐为电子受体氧化氨氮，其硝酸盐还原速率高于亚硝酸盐还原速率，从而产生亚硝酸盐供厌氧氨氧化反应使用。宏基因组学分析表明，缺氧生物膜中的优势种为黑尾水雉和斯氏水雉。在缺氧生物膜中检测到参与厌氧氨氧化过程代谢的基因。缺氧生物膜中硝酸还原酶的丰度（73360 hits）远高于亚硝酸还原酶（13114 hits）。该系统可以很容易地与高速率活性污泥技术集成，该技术产生具有低C/N比的流出物。虽然这种新设计比目前研究的亚硝化/厌氧氨氧化工艺多消耗21%的氧气，但亚硝酸盐生产工艺似乎更稳定。
Achieving maintream anammox is critical for energy-neutral sewage treatment. This study presents a new way to achieve mainstream anammox, which couples anammox with denitratation (nitrate reduction to nitrite) instead of nitritation (ammonium oxidation to nitrite). An anoxic/oxic (A/O) biofilm system treating systhetic domestic wastewater was used to demonstrate this concept for over 400 days. This A/O biofilm system achieved a total nitrogen (TN) removal efficiency of 80 ± 4% from the influent with a low C/N ratio of 2.6 and a TN concentration of 60.5 mg/L. Nitrogen removal via anammox was found to account for 70% of dinitrogen production in the anoxic reactor. Batch tests confirmed that the anoxic biofilm could oxidize ammonium using nitrite as electron acceptor, and that it had a higher nitrate reduction rate than the nitrite reduction rate, thus producing nitrite for the anammox reaction. Metagenomic analysis showed that Candidatus Jettenia caeni and Candidatus Kuenenia stuttgartiensis were the top two dominant species in anoxic biofilm. Genes involved in the metabolism of the anammox process were detected in anoxic biofilm. The abundance of nitrate reductase (73360 hits) was much higher than nitrite reductase (13114 hits) in anoxic biofilm. This system can be easily integrated with the high-rate activated sludge technology, which produces an effluent with a low C/N ratio. While this new design consumes 21% more oxygen in comparison to the currently studied nitritation/anammox process, the nitrite-producing process appears to be more stable.