Atypical E2F activity coordinates PHR1 photolyase gene transcription with endoreduplication onset
Atypical E2F activity coordinates PHR1 photolyase gene transcription with endoreduplication onset
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DOI：
10.1038/emboj.2010.313
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发表时间：
2011-01-19
期刊：
影响因子：
11.4
通讯作者：
De Veylder, Lieven
中科院分区：
文献类型：
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作者：
Radziejwoski, Amandine;Vlieghe, Kobe;De Veylder, Lieven
由于它们的固着生活方式，植物进化出了适应恶劣环境条件的能力。压力适应的一个重要方面涉及细胞周期的重新编程以确保最佳生长。非典型E2 F转录因子DP-E2 F-like 1（E2 Fe/DEL 1）是细胞内循环启动的重要调节因子。在这里，E2 Fe/DEL 1的一个新的作用被确定为II型环丁烷嘧啶二聚体-光解酶DNA修复基因PHR 1的转录抑制因子。在紫外线-B（UV-B）处理，植物敲除E2 Fe/DEL 1有改善的DNA修复能力相比，对照植物，而那些过表达它表现不佳。更好的DNA修复使E2 Fe/DEL 1敲除植物比对照植物更快地恢复核内复制，从而通过倍性依赖性细胞生长补偿胁迫诱导的细胞数量减少，从而以这种方式增强对UV-B辐射的抵抗力。由于UV-B处理后E2 Fe/DEL 1水平降低，我们推测PHR 1的协同转录诱导与核内复制的启动有助于植物对UV-B胁迫的适应。The EMBO Journal（2011）30，355-363. doi：10.1038/doj.2010.313; 2010年12月3日在线发布
Because of their sessile life style, plants have, evolved the ability to adjust to environmentally harsh conditions. An important aspect of stress adaptation involves the reprogramming of the cell cycle to ensure optimal growth. The atypical E2F transcription factor DP-E2F-like 1 (E2Fe/DEL1) had been found previously to be an important regulator of the endocycle onset. Here, a novel role for E2Fe/DEL1 was identified as a transcriptional repressor of the type-II cyclobutane pyrimidine dimer-photolyase DNA repair gene PHR1. Upon ultraviolet-B (UV-B) treatment, plants knocked out for E2Fe/DEL1 had improved DNA repair abilities when compared with control plants, whereas those overexpressing it performed less well. Better DNA repair allowed E2Fe/DEL1 knockout plants to resume endoreduplication faster than control plants, contributing in this manner to UV-B radiation resistance by compensating the stress-induced reduction in cell number by ploidy-dependent cell growth. As E2Fe/DEL1 levels decreased upon UV-B treatment, we hypothesize that the coordinated transcriptional induction of PHR1 with the endoreduplication onset contributes to the adaptation of plants exposed to UV-B stress. The EMBO Journal (2011) 30, 355-363. doi:10.1038/emboj.2010.313; Published online 3 December 2010