Inhibitory effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells
Inhibitory effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells
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DOI：
10.1039/c1fo10209e
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Yen, Gow-Chin
中科院分区：
文献类型：
--
作者：
Hsu, Chin-Lin;Lin, Yu-Jyun;Yen, Gow-Chin
本研究的目的是研究Garcinol和Pterostilbene对3 T3-L1细胞增殖和脂肪形成的影响。结果表明，加辛和紫檀芪可抑制3 T3-L1前脂肪细胞的生长，并使细胞周期阻滞于G2/M期。在脂肪细胞分化过程中，加辛和紫檀芪都对脂肪滴的形成和三酰甘油的积累有抑制作用。结果表明，与对照组相比，藤黄酚和紫檀芪对甘油-3-磷酸脱氢酶（GPDH）活性的抑制率分别为97.8%和61.5%。Garcinol和pterostilbene均显着减弱3 T3-L1脂肪细胞分化过程中PPAR γ和C/EBP α的蛋白表达。此外，Garcinol和Pterostilbene引起在3 T3-L1脂肪细胞分化阶段的脂质积累的抑制。在3 T3-L1脂肪细胞分化过程中，Garcinol和Pterostilbene均能显著上调脂联素基因表达，下调瘦素、脂肪酸合成酶（FAS）和脂肪酸合成酶（Leptin）基因表达。在3 T3-L1脂肪细胞中，加辛醇可显著下调PPAR γ和FAS的蛋白表达，上调脂肪甘油三酯脂酶（ATGL）和脂联素的蛋白表达。Garcinol还显著上调脂联素基因表达，下调瘦素和FAS基因表达。这些结果表明，Garcinol和紫檀芪对前脂肪细胞和脂肪细胞具有抗成脂作用。
The aim of this work was to study the effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells. The results showed that garcinol and pterostilbene decreased the cell population growth and caused cell cycle arrest at the G2/M phase in 3T3-L1 preadipocytes. During adipocyte differentiation, both garcinol and pterostilbene had inhibitory effects on fat droplet formation and triacylglycerol accumulation. The data indicated that garcinol and pterostilbene could inhibit the glycerol-3-phosphate dehydrogenase (GPDH) activity by 97.8 and 61.5%, respectively, as compared to the control. Both garcinol and pterostilbene significantly attenuated the protein expressions of PPAR gamma and C/EBP alpha during 3T3-L1 adipocyte differentiation. Moreover, garcinol and pterostilbene caused an inhibition of lipid accumulation in the 3T3-L1 adipocyte differentiation phase. Garcinol and pterostilbene also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin, resistin, and fatty acid synthase (FAS) in 3T3-L1 adipocyte differentiation. In 3T3-L1 adipocytes, garcinol significantly down-regulated the protein expressions of PPAR gamma and FAS as well as up-regulated the protein expressions of adipose triglyceride lipase (ATGL) and adiponectin. Garcinol also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin and FAS. These results suggest that garcinol and pterostilbene have anti-adipogenic effects on preadipocytes and adipocytes.