Relationship between enteric neurons and interstitial cells in the primate gastrointestinal tract
Relationship between enteric neurons and interstitial cells in the primate gastrointestinal tract
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DOI：
10.1111/j.1365-2982.2012.01975.x
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发表时间：
2012-09-01
影响因子：
3.5
通讯作者：
Ward, S. M.
中科院分区：
文献类型：
--
作者：
Blair, P. J.;Bayguinov, Y.;Ward, S. M.
背景形态学研究表明，肠神经末梢与肌内ICC(ICC-IM)之间存在密切的解剖关系，支持ICC-IM作为肠运动神经传递的中介作用。最近，第二种间质细胞以前被描述为成纤维细胞样但现在可以通过血小板衍生生长因子受体-a的表达来鉴定，也被认为与啮齿类动物的肠道神经传递有关。本研究旨在确定肠神经纤维是否与整个灵长类胃肠道的ICC和PDGFRA+细胞形成了密切的解剖关系。方法应用免疫组织化学和激光共聚焦显微镜技术，观察猴胃肠道内兴奋性和抑制性运动神经元、ICC和PDGFRA+细胞之间的关系。关键结果是泛神经元标记物。用蛋白基因产物9.5(PGP9.5)标记所有肠神经细胞，用P物质(SubP)和神经元型一氧化氮合酶(NNOS)分别标记兴奋性和抑制性神经元。Kit双标结果显示，在胃、小肠和结肠(带带区和带间区)，这两类神经纤维与ICC-IM紧密对置，但在肌间神经丛(ICC-My)水平上与ICC不同。静脉曲张的肠神经纤维与ICC-IM的联系距离可达250微米，兴奋性神经纤维和抑制性神经纤维也与PDGFRA+细胞紧密接触，分布于灵长类动物胃肠道。结论：食蟹猴胃肠道内ICC-IM和PDGFRA+细胞与肠神经纤维的密切解剖关系，为这两类间质细胞在灵长类动物中发挥与啮齿动物模型相似的生理功能提供了形态学证据。
Background Morphological studies have revealed a close anatomical relationship between enteric nerve terminals and intramuscular ICC (ICC-IM) which supports a role for ICC-IM as intermediaries in enteric motor neurotransmission. Recently, a second type of interstitial cell previously described as fibroblast-like but can now be identified by platelet-derived growth factor receptor-a expression, has also been implicated in enteric neurotransmission in rodents. The present study was performed to determine if enteric nerve fibers form close anatomical relationships with ICC and PDGFRa+ cells throughout the primate GI tract. Methods Immunohistochemical experiments and confocal microscopy were performed to examine the relationship between excitatory and inhibitory motor neurons, ICC and PDGFRa+ cells throughout the monkey GI tract. Key Results The pan neuronal marker. Protein gene product 9.5 (PGP9.5) was used to label all enteric neurons and substance-P (sub-P) and neuronal nitric oxide synthase (nNOS) to label excitatory and inhibitory neurons, respectively. Double labeling with Kit revealed that both classes of nerve fibers were closely apposed with ICC-IM in the stomach, small intestine and colon (taenia and inter-taenia regions), but not with ICC at the level of the myenteric plexus (ICC-MY). Varicose enteric nerve fibers were closely associated with ICC-IM for distances up to 250 mu m. Both excitatory and inhibitory nerve fibers were also closely apposed to PDGFRa+ cells throughout the primate GI tract. Conclusions & Inferences The close anatomical relationship between enteric nerve fibers and ICC-IM and PDGFRa+ cells throughout the GI tract of the Cynomolgus monkey provides morphological evidence that these two classes of interstitial cells may provide a similar physiological function in primates as has been attributed in rodent animal models.