Method for screening peptide fragment ion mass spectra prior to database searching
Method for screening peptide fragment ion mass spectra prior to database searching
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DOI：
10.1016/s1044-0305(00)00097-0
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发表时间：
2000-05-01
影响因子：
3.2
通讯作者：
Lee, TD
中科院分区：
文献类型：
--
作者：
Moore, RE;Young, MK;Lee, TD
描述了一种用于在数据库搜索蛋白质鉴定之前筛选肽的碎片离子谱的方法。用Perl编程语言编写的软件例程用于分析来自先前Sequest数据库检索的数据，并开发一组统计描述符，其可用于鉴定在数据库检索中不太可能产生有用结果的光谱。第二个Perl程序使用进化算法来优化每个统计描述符的标准，并生成用于确定光谱质量的公式。第三个Perl程序使用该公式筛选来自四个独立液相色谱串联质谱运行的数据集。平均而言，筛选程序的使用减少了1/2的数据库检索所需的时间，几乎没有从数据库检索结果中丢失有用的信息。(C)2000年美国质谱学会。
A methodology is described for screening fragment ion spectra of peptides prior to database searching for protein identification. A software routine written in the Perl programming language was used to analyze data from previous Sequest database searches and develop a set of statistical descriptors that could be used to identify spectra not likely to yield useful results in a database search. A second Perl program used an evolutionary algorithm to optimize the criteria for each statistical descriptor and generate a formula for determining spectral quality. This formula was used by a third Perl program to screen data sets from four independent liquid chromatography tandem mass spectrometry runs. On the average, use of the screening program reduced the time required for a database search by 1/2 with little loss of useful information from the database search results. (C) 2000 American Society for Mass Spectrometry.