Invited Article: Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces
Invited Article: Heterodyne dual-polarization epi-detected CARS microscopy for chemical and topographic imaging of interfaces
复制标题
DOI：
10.1063/1.5027256
复制
发表时间：
2018-09-01
期刊：
影响因子：
5.6
通讯作者：
Borri, Paola
中科院分区：
文献类型：
--
作者：
Langbein, Wolfgang;Regan, David;Borri, Paola
相干拉曼散射（CRS）在过去十年中作为一种强大的多光子显微镜技术出现，其提供具有高三维空间分辨率的真实的时间的化学特异性无标记成像。CRS显微镜的许多技术实现已被提出来去除，抑制，或占非共振背景的非线性磁化率，复杂的光谱分析和降低图像对比度。在这里，我们展示了相干反斯托克斯拉曼散射显微镜使用双偏振平衡外差检测epigeometry（eH-CARS），提供背景无化学特定的图像对比度的纳米粒子和接口，散粒噪声有限的检测，和相位灵敏度。我们显示的灵敏度和选择性的eH-CARS相比，前向汽车和受激拉曼散射聚苯乙烯珠在琼脂糖凝胶。作为一个重要的生物相关的应用，我们证明了eH-CARS成像的个人脂质双层高对比度和地形敏感性。(C)2018年作者。
Coherent Raman Scattering (CRS) has emerged in the last decade as a powerful multiphoton microscopy technique offering chemically specific label-free imaging in real time with high three-dimensional spatial resolution. Many technical realizations of CRS microscopy have been proposed to remove, suppress, or account for the nonresonant background in the nonlinear susceptibility which complicates spectral analysis and reduces image contrast. Here, we demonstrate coherent anti-Stokes Raman scattering microscopy using a dual-polarization balanced heterodyne detection in epigeometry (eH-CARS), providing background-free chemically specific image contrast for nanoparticles and interfaces, shot-noise limited detection, and phase sensitivity. We show the sensitivity and selectivity of eH-CARS in comparison with forward CARS and stimulated Raman scattering on polystyrene beads in agarose gel. As an important biologically relevant application, we demonstrate eH-CARS imaging of individual lipid bilayers with high contrast and topographic sensitivity. (C) 2018 Author(s).