Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients
Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients
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DOI：
10.1093/humrep/deh768
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发表时间：
2005-06-01
影响因子：
6.1
通讯作者：
Suikkari, AM
中科院分区：
文献类型：
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作者：
Söderström-Anttila, V;Mäkinen, S;Suikkari, AM
背景：目的是为广泛的患者开发一种体外卵母细胞成熟（IVM）方法。方法：未成熟卵母细胞回收（IOC） 239个周期，无激素启动。周期正常、正常或多囊卵巢（PCO）和无排卵周期的PCOS患者纳入研究对象。成熟卵母细胞的受精根据精子质量选择人工授精或胞浆内单精子注射（ICSI）。结果：122个ivf - ivf周期共收集到971个未成熟卵母细胞（平均8.0 +/- 5.2），117个ivf - icsi周期共收集到851个卵母细胞（平均7.3 +/- 4.4）。授精后卵母细胞成熟率为62.6%，受精率为37.7%，ICSI后卵母细胞成熟率为53.9%，受精率为69.3%。平均移植胚胎数为1.6个。IVM-IVF和IVM-ICSI的临床妊娠率分别为23.8%和17.1% （ns）。ivf - ivf的着床率（24.2%）高于ivf - icsi (14.8%) （P < 0.05）。结论：IVM卵母细胞的授精功能良好，导致IVM- ivf和IVM- icsi的每IOC妊娠率相当。周期规律和PCO(S)患者一次只移植一个或两个胚胎都可以获得良好的妊娠结果。
BACKGROUND: The purpose has been to develop an in vitro oocyte maturation (IVM) method for a wide range of patients. METHODS: A total of 239 cycles with immature oocyte retrieval (IOC) were carried out without hormonal priming. Patients with regular cycles and normal or polycystic ovaries (PCO) and anovulatory cycles with PCOS were included. Insemination or intracytoplasmic sperm injection (ICSI) according to sperm quality was alternatively used in fertilization of the matured oocytes. RESULTS: A total of 971 immature oocytes (mean 8.0 +/- 5.2) were collected in 122 IVM-IVF cycles and 851 oocytes (mean 7.3 +/- 4.4) in 117 IVM-ICSI cycles. The oocyte maturation and fertilization rate was 62.6% and 37.7% after insemination, and 53.9% and 69.3% after ICSI, respectively. The mean number of embryos transferred was 1.6. Clinical pregnancy rate per IOC was 23.8% in IVM-IVF and 17.1% in IVM-ICSI (ns). Implantation rate was higher in IVM-IVF (24.2%) than in IVM-ICSI (14.8%) (P < 0.05). CONCLUSIONS: Insemination of IVM oocytes functions well, resulting in comparable pregnancy rates per IOC between IVM-IVF and IVM-ICSI. Good pregnancy results can be achieved both in patients with regular cycles and with PCO(S) by transferring only one or two embryos at a time.