Isolation of a Ca2+‐Dependent Actin‐Fragmenting Protein from Brain, Spinal Cord, and Cultured Neurones
Isolation of a Ca2+‐Dependent Actin‐Fragmenting Protein from Brain, Spinal Cord, and Cultured Neurones
复制标题
从大脑、脊髓和培养神经元中分离 Ca2+ 依赖性肌动蛋白片段蛋白
作者：
T. Petrucci;C. Thomas;D. Bray
Extracts of ox spinal cord and chicken brain were fractionated by ion‐exchange chromatography and assayed for their ability to reduce the viscosity of muscle F‐actin solutions. Two distinct peaks of activity were obtained, one of which was further purified by affinity chromatography on a DNAase‐actin Sepharose column. Following molecular exclusion chromatography, the actin component appeared as a complex of 1 molecule of a protein with molecular weight 90,000 and 2 molecules of actin (42,000). This tightly bound complex was resistant to most methods of protein separation, but was resolvable into its component proteins by sodium dodecyl sulphate acrylamide gel electrophoresis. The protein of molecular weight 90,000 could be eluted from such a gel in a fully active form. The activity of the protein from ox spinal cord was closely similar to that of gelsolin, an actin‐fragmenting protein originally isolated from rabbit lung macrophages. Like gelsolin, the protein from ox spinal cord produced fragmentation of muscle F‐actin filaments at Ca2+ concentrations greater than 10‐7M, and had a nucleating effect on the polymerisation of muscle actin; the latter was measured most easily by the enhancement of fluorescence of muscle actin conjugated to N‐(1‐pyrenyl)iodoacetamide. Nucleation was more effective in the presence of Ca2+, but also occurred in its absence, and the same was true of complex formation between the 90,000 protein and muscle G‐actin. On the basis of its actin‐fragmenting activity, we estimate that the 90,000 molecular weight protein constitutes 0.2% of the protein initially extracted from ox spinal cord. A very similar protein, indistinguishable in its action on actin but containing variable amounts of a protein of molecular weight 85,000 as well as 90,000, was isolated from chicken brain. A similar protein was also detected in pure cultures of sympathetic neurones by enrichment on a DNAase‐actin affinity column and by immune blotting and by immuno‐fluorescence. We conclude that a protein similar, if not identical to macrophage gelsolin is present in neurones and that it probably plays a part in the actin‐based movements of these cells.
凝溶胶蛋白（一种 Ca2 激活的巨噬细胞调节蛋白）的纯化和结构特性。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yin,HL;Stossel,TP
通讯作者：
Stossel,TP