Gene expression in Escherichia coli biofilms
Gene expression in Escherichia coli biofilms
复制标题
DOI：
10.1007/s00253-003-1517-y
复制
发表时间：
2004-05-01
影响因子：
5
通讯作者：
Wood, TK
中科院分区：
文献类型：
--
作者：
Ren, D;Bedzyk, LA;Wood, TK
利用基因芯片技术研究了大肠杆菌JM 109和K12生物膜的基因表达谱。摇瓶中的玻璃棉和连续反应器中的低碳钢1010板都用于产生生物膜。对于生长在玻璃棉上的生物膜，有22个基因被显著诱导（小于或等于0.05），包括几个应激反应基因（hslS、hslT、hha和soxS）、I型菌毛（fimG）、代谢（metK）和11个功能未知的基因（ybaJ、ychM、yefM、ygfA、b1060、b1112、b2377、b3022、b1373、b1601和b0836）。DNA微阵列结果与RNA斑点杂交相结合。对于在低碳钢板上生长的生物膜，DNA微阵列数据显示，在0.05/h的比生长速率下，在连续反应器中5天后的成熟生物膜与悬浮细胞相比没有表现出差异基因表达，尽管基因在0.03/h下被诱导。目前的研究表明，生物膜基因的表达与环境条件密切相关，应激基因参与了E。coli JM 109生物膜形成。
DNA microarrays were used to study the gene expression profile of Escherichia coli JM109 and K12 biofilms. Both glass wool in shake flasks and mild steel 1010 plates in continuous reactors were used to create the biofilms. For the biofilms grown on glass wool, 22 genes were induced significantly (pless than or equal to0.05) compared to suspension cells, including several genes for the stress response (hslS, hslT, hha, and soxS), type I fimbriae (fimG), metabolism (metK), and 11 genes of unknown function (ybaJ, ychM, yefM, ygfA, b1060, b1112, b2377, b3022, b1373, b1601, and b0836). The DNA microarray results were corroborated with RNA dot blotting. For the biofilm grown on mild steel plates, the DNA microarray data showed that, at a specific growth rate of 0.05/h, the mature biofilm after 5 days in the continuous reactors did not exhibit differential gene expression compared to suspension cells although genes were induced at 0.03/h. The present study suggests that biofilm gene expression is strongly associated with environmental conditions and that stress genes are involved in E. coli JM109 biofilm formation.