Cold-storage preservation of the canine and rat pancreas prior to islet isolation.
Cold-storage preservation of the canine and rat pancreas prior to islet isolation.
复制标题
在胰岛分离之前冷藏保存犬和大鼠胰腺。
DOI：
10.1097/00007890-198901000-00007
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发表时间：
1989
期刊：
影响因子：
6.2
通讯作者：
Sutherland,DE
中科院分区：
文献类型：
--
作者：
Munn,SR;Kaufman,DB;Field,MJ;Viste,AB;Sutherland,DE
材料和方法动物模型。使用的是经过兽医检验的近交狗和市面上可买到的Lewis(RT 1“)大鼠。前瞻性方案用于确定安乐死的必要性和时机。此外，兽医定期检查以评估和治疗并存疾病。犬：在全麻下施行保留十二指肠的胰腺切除术(8只)(硫代戊酸钠15 mg/kg；阿托品1 mg；琥珀胆碱0.5 mg/kg；然后插管、换气，间断静脉注射芬太尼和氟哌利多维持)。全胰腺切除治疗糖尿病的疗效已被证实(6)。大鼠：移植前1周静脉注射链脲佐菌素(60 mg/kg)，平均年龄12周，平均体重250g。只有当非空腹血糖为375 mg/dl时，糖尿病才被认为是诱发的。保存解决方案。犬的节段性自体移植研究表明，目前可用的最有效的胰腺保存液是SGF-III、SGF-IV和UW-1，其成分如表1所示。当作为立即吻合血管的移植物移植时，这三种方法都证明了成功保存胰腺长达72小时的能力(9，10)。UW-1在Lewis大鼠的胰腺十二指肠移植物冷藏中保存24小时，但不是48小时，也是令人满意的。在冷藏1小时、24小时或48小时后，将同种异体移植物移植到链脲佐菌素诱导的糖尿病患者的受者体内。糖尿病反转率分别为8/10、4/5和0/4。前两组的失败与麻醉并发症或出血有关，而不是与持续性高血糖有关。虽然这三种溶液都可以在犬模型中进行测试，但制造Lewis大鼠SGF-III或SGF-IV是不可行的，因此在该模型中只测试了UW-1。
MATERIALS AND METHODS Animals. Veterinarian-inspected outbred dogs and commercially available Lewis (RT 1") rats were used. Prospective protocols were used to determine the necessity for, and timing of euthanasia. In addition, there were regular inspections by veterinarians to evaluate and treat intercurrent illnesses.Diabetes induction. Dogs: A duodenum-preserving pancreatectomy (8) was carried out under general anaesthesia (thiamylal sodium, 15 mg/kg; atropine, 1 mg; and succinylcholine, 0.5 mg/kg, for induction; followed by intubation, ventilation, and intermittent intravenous fen-tanyl and droperidol, for maintenance). The efficacy of total pancreatectomy for diabetes induction had previously been demonstrated (6). Rats: A single dose of streptozotocin (60 mg/kg) was given intravenously to animals of average age 12 weeks and average weight 250 g, one week prior to transplantation. Diabetes was considered induced only if the nonfasting blood glucose was> 375 mg/dl. Preservation solutions. Canine segmental autograft studies have indicated that the most effective of the currently available pancreatic preservation solutions are SGF-III, SGF-IV, and UW-1, the contents of which are described in Table 1. All three have demonstrated the ability to successfully preserve the pancreas for up to 72 hr, when transplanted as an immediately vascularized graft (9, 10). UW-1 was also satisfactory for pancreaticoduodenal graft preservation in the Lewis rat for 24 hr, but not 48 hr, of cold storage. This was shown by transplanting isografts into recipients with streptozotocin-induced diabetes after< 1 hr, 24 hr, or 48 hr of cold storage. The rate of diabetic reversal was 8/10, 4/5, and 0/4, respectively. The failures in the first two groups were related to anesthetic complications or hemorrhage rather than to persisting hyperglycemia. While all three solutions could be tested in the canine model, it was not feasible to manufacture Lewis rat SGF-III or SGF-IV, so only UW-1 was tested in this model.