Gut microbiota protects honey bees (Apis mellifera L.) against polystyrene microplastics exposure risks.
Gut microbiota protects honey bees (Apis mellifera L.) against polystyrene microplastics exposure risks.
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DOI：
10.1016/j.jhazmat.2020.123828
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发表时间：
2021-01
影响因子：
13.6
通讯作者：
Kai Wang;Jiahuan Li;Liuwei Zhao;Xiyan Mu;Chen Wang;Miao Wang;X. Xue;Suzhen Qi;Liming Wu
中科院分区：
文献类型：
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作者：
Kai Wang;Jiahuan Li;Liuwei Zhao;Xiyan Mu;Chen Wang;Miao Wang;X. Xue;Suzhen Qi;Liming Wu
微塑料污染不仅是海洋中的一个紧迫的环境问题，也是陆地生态系统的一个紧迫问题。然而，人们对它对传粉者的潜在影响知之甚少。在这里，我们报告了25 μ m直径的球形聚苯乙烯微塑料（PS-MPs）单独或与抗生素四环素组合对蜜蜂（意大利蜜蜂）的影响。在实验室我们注意到PS-MPs暴露14 d具有亚致死效应，在三种不同处理（0.5，5和50 mg/L）中具有低死亡率（高达1.6%），并且与对照蜜蜂相比体重增加没有变化。然而，PS-MPs暴露导致蜜蜂肠道微生物群的α多样性显着下降，伴随着核心微生物种群结构的变化。此外，PS-MP导致肠道中抗氧化（Cat）、解毒（CypQ 1和GstS 3）和免疫系统相关基因（Domeless、Hopscotch和Symplekin）表达的改变。更有趣的是，我们观察到PS-MP在后肠内积累和降解，并与肠道细菌相互作用。使用四环素消耗正常肠道微生物群大大增加了微塑料的致命性。这些结果为未来研究其他昆虫中微塑料与微生物组相互作用提供了资源，也为了解微塑料在陆地生态系统中的潜在影响提供了线索。
Microplastic contamination is not only a pressing environmental concern in oceans, but also terrestrial ecosystems. However, little is known about its potential impacts on pollinators. Here, we reported the effects of 25 μm-diameter spherical polystyrene microplastics (PS-MPs) alone or in combination with the antibiotic tetracycline on honey bees (Apis melliferaL.) in the laboratory. We noticed that PS-MPs exposure for 14 d had sublethal effects, with low mortalities (up to 1.6 %) across three different treatments (0.5, 5, and 50 mg/L) and no changes to the body weight gains compared to the control bees. Nevertheless, PS-MPs exposure led to significant decreases in the α-diversity of bees’ gut microbiota accompanied by changes to the core microbial population structure. Additionally, PS-MPs lead to alterations in the expression of antioxidative (Cat), detoxification (CypQ1andGstS3), and immune system-related genes (Domeless,Hopscotch, andSymplekin) in guts. More interestingly, we observed that PS-MPs accumulated and degraded inside of the hindgut and interacted with gut bacteria. The depletion of the normal gut microbiota using tetracycline dramatically increased the lethality of microplastics. These results provide a resource for future research on microplastic-microbiome interactions in other insects and also shed light on understanding the potential effects of microplastics in terrestrial ecosystems.