Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions.
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions.
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DOI：
10.3791/53911
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发表时间：
2016-06-14
期刊：
影响因子：
--
通讯作者：
Yang Z
中科院分区：
文献类型：
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作者：
Rao W;Pan N;Yang Z
环境条件下的质谱成像（MSI）和原位单细胞质谱（SCMS）分析是两个新兴的领域，其对于来自生物样品的生物分子的详细质谱（MS）分析具有巨大潜力。单探针是一种集成了采样和电离功能的小型化设备，能够进行环境MSI和原位SCMS分析。对于环境MSI，单探针使用表面微萃取持续进行样品的MS分析，该技术允许从生物样品（如小鼠大脑和肾脏切片）中创建具有高空间分辨率（8.5 µm）的MS图像。Ambient MSI的优点是在分析之前几乎不需要样品制备，这减少了数据采集中存在的潜在伪影的量，并允许对待采集的样品进行更具代表性的分析。对于原位SCMS，由于采样尖端尺寸小（< 10 µm），单探针尖端可以直接插入活的真核细胞（如HeLa细胞）中，并且该技术能够近实时地检测单个细胞内的各种代谢物。SCMS能够在单细胞水平上获得更高灵敏度和准确性的化学信息，这可以在比以前更基础的水平上提高我们对生物过程的理解。单探针装置可以潜在地与各种质谱仪耦合，用于广泛的MSI和SCMS研究。
Mass spectrometry imaging (MSI) and in-situ single cell mass spectrometry (SCMS) analysis under ambient conditions are two emerging fields with great potential for the detailed mass spectrometry (MS) analysis of biomolecules from biological samples. The single-probe, a miniaturized device with integrated sampling and ionization capabilities, is capable of performing both ambient MSI and in-situ SCMS analysis. For ambient MSI, the single-probe uses surface micro-extraction to continually conduct MS analysis of the sample, and this technique allows the creation of MS images with high spatial resolution (8.5 µm) from biological samples such as mouse brain and kidney sections. Ambient MSI has the advantage that little to no sample preparation is needed before the analysis, which reduces the amount of potential artifacts present in data acquisition and allows a more representative analysis of the sample to be acquired. For in-situ SCMS, the single-probe tip can be directly inserted into live eukaryotic cells such as HeLa cells, due to the small sampling tip size (< 10 µm), and this technique is capable of detecting a wide range of metabolites inside individual cells at near real-time. SCMS enables a greater sensitivity and accuracy of chemical information to be acquired at the single cell level, which could improve our understanding of biological processes at a more fundamental level than previously possible. The single-probe device can be potentially coupled with a variety of mass spectrometers for broad ranges of MSI and SCMS studies.