Transfer RNA-derived fragments as novel biomarkers of the onset and progression of gastric cancer
Transfer RNA-derived fragments as novel biomarkers of the onset and progression of gastric cancer
复制标题
DOI：
10.1177/15353702231179415
复制
发表时间：
2023-06
影响因子：
3.2
通讯作者：
Yaoyao Xie;Shuangshuang Zhang;Xiuchong Yu;Guoliang Ye;Junming Guo
中科院分区：
文献类型：
--
作者：
Yaoyao Xie;Shuangshuang Zhang;Xiuchong Yu;Guoliang Ye;Junming Guo
胃癌（GC）是一种恶性程度特别高的疾病；因此，早期诊断和治疗尤为重要。转移 RNA 衍生的小 RNA (tsRNA) 与多种癌症的发生和进展有关。因此，本研究的目的是探讨tRF-18-79MP9P04（以前称为tRF-5026a）在GC发生和进展中的作用。对健康对照胃粘膜标本和不同阶段GC患者血浆样本中tRF-18-79MP9P04的表达水平进行定量。结果显示，GC早期和晚期血浆tRF-18-79MP9P04水平显着降低。核质分离实验结果发现tRF-18-79MP9P04定位于GC细胞的细胞核。高通量转录组测序鉴定了GC细胞中受tRF-18-79MP9P04调控的基因，并通过生物信息学预测了tRF-18-79MP9P04的功能。总的来说，本研究的结果表明 tRF-18-79MP9P04 可作为 GC 早期诊断的非侵入性生物标志物，并且与角化、I 型干扰素信号通路、RNA 聚合酶 II 活性和 DNA 结合有关。
Gastric cancer (GC) is a particularly malignant disease; thus, early diagnosis and treatment are especially important. Transfer RNA-derived small RNAs (tsRNAs) have been implicated in the onset and progression of various cancers. Therefore, the aim of this study was to explore the role of tRF-18-79MP9P04 (previously named tRF-5026a) in the onset and progression of GC. Expression levels of tRF-18-79MP9P04 were quantified in gastric mucosa specimens of healthy controls and plasma samples of patients with different stages of GC. The results showed that plasma levels of tRF-18-79MP9P04 were significantly decreased in the early and advanced stages of GC. The results of the nucleocytoplasmic separation assay found that tRF-18-79MP9P04 was localized in the nuclei of GC cells. High-throughput transcriptome sequencing identified genes regulated by tRF-18-79MP9P04 in GC cells, and the function of tRF-18-79MP9P04 was predicted by bioinformatics. Collectively, the findings of this study suggest that tRF-18-79MP9P04 would be useful as non-invasive biomarker for early diagnosis of GC and is related to cornification, the type I interferon signaling pathway, RNA polymerase II activities, and DNA binding.