Interaction and reconstitution of carboxyl-terminal-shortened B chains with the intact A chain of insulin.
Interaction and reconstitution of carboxyl-terminal-shortened B chains with the intact A chain of insulin.
复制标题
羧基末端缩短的 B 链与完整的胰岛素 A 链的相互作用和重建。
DOI：
10.1021/bi00366a052
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Tsou,CL
中科院分区：
文献类型：
--
作者：
Wang,CC;Tsou,CL
材料和方法试剂。猪胰岛素是Novo的产品。胃蛋白酶由E.Merck公司获得。DTNB、TPCK-胰酶、胶原酶(II型)和白蛋白(牛，组分V)是Sigma产物。乙酰酪氨酸乙酯，产自上海东方生化。DTT来自SERVA，巯基乙醇来自福尔卡，TFA来自皮尔斯。尿素是一种本地产品，根据Morangos和1缩写进行了两次重结晶：乙酸乙酯，乙酰酪氨酸乙酯；CD，圆二色；DP，去八肽(B26-30)；DO，去八肽(B23-30)；DTNB，5，5‘-二硫双(2-硝基苯甲酸)；DTT，二硫苏糖醇；EDTA，乙二胺四乙酸；高效液相色谱法；TFA，三氟三氟乙酸；TPCK，LL-[(对甲苯磺基)氨基]-2-苯甲基氯甲酮；is-HCl，三(羟甲基)氨基甲烷盐酸盐。
Materials and MethodsReagents. Porcine insulin was a product of Novo. Pepsin was obtained from E. Merck. DTNB, TPCK-trypsin, collagenase (type II), and albumin (Bovine, fraction V) were Sigma products. Acetyltyrosine ethyl ester was from Dongfong Biochemicals, Shanghai. DTT was from Serva, mercapto-ethanol from Fluka, and TFA from Pierce. Urea was a local product twice recrystallized according to Morangos and1 Abbreviations: ATEE, acetyltyrosine ethyl ester; CD, circular dichroism; DP, despentapeptide (B26-30); DO, desoctapeptide (B23-30); DTNB, 5, 5'-dithiobis (2-nitrobenzoicacid); DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; HPLC, high-performance liquid chro-matography; TFA, trifluoroacetic acid; TPCK, Ll-[(p-toluene-sulfonyl) amino]-2-phenylmethyl chloromethyl ketone; Tris-HCl, tris-(hydroxymethyl) aminomethane hydrochloride.