Enhanced one-carbon flux towards DNA methylation: Effect of dietary methyl supplements against γ-radiation-induced epigenetic modifications
Enhanced one-carbon flux towards DNA methylation: Effect of dietary methyl supplements against γ-radiation-induced epigenetic modifications
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DOI：
10.1016/j.cbi.2009.11.010
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发表时间：
2010-02-12
影响因子：
5.1
通讯作者：
Devasagayam, Thomas Paul Asir
中科院分区：
文献类型：
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作者：
Batra, Vipen;Sridhar, Swathi;Devasagayam, Thomas Paul Asir
辐射暴露对核电站和其他辐射相关行业的工人构成了重大风险。在此背景下，我们证明了伽玛辐射是一种有效的DNA去甲基化试剂，其损害作用可以通过饮食中的甲基补充剂(叶酸、胆碱和维生素B12)来最小化。为了阐明可能的潜在机制(S)，雄性瑞士小鼠维持正常对照饲料(NCD)和添加甲基的饲料(MSD)。在NCD和MSD饮食方案的2周后，我们将动物暴露于伽马辐射(2，4和6GY)，并调查下游代谢物的分布和一碳(C-1)助熔剂产生酶的活性水平。经MSD喂养和照射的动物，肝脏叶酸水平升高(P
Radiation exposure poses a major risk for workers in the nuclear power plants and other radiation related industry. In this context, we demonstrate that gamma-radiation is an efficient DNA demethylating agent and its injurious effect can be minimized by dietary methyl Supplements (folate, choline and vitamin B12). To elucidate the possible underlying mechanism(s), male Swiss mice were maintained on normal control diet (NCD) and methyl-supplemented diet (MSD). After 2 weeks of NCD and MSD dietary regimen, we exposed the animals to gamma-radiation (2, 4 and 6Gy) and investigated the profile of downstream metabolites and activity levels of one-carbon (C-1) flux generating enzymes. In MSD fed and irradiated animals, hepatic folate levels increased (P