Complete genome characterization of Rocio virus (Flavivirus:Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in Sao Paulo state
Complete genome characterization of Rocio virus (Flavivirus:Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in Sao Paulo state
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DOI：
10.1099/vir.0.82883-0
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发表时间：
2007-08-01
影响因子：
3.8
通讯作者：
Kuno, Goro
中科院分区：
文献类型：
--
作者：
Medeiros, Daniele B. A.;Nunes, Marcio R. T.;Kuno, Goro
在南美国家具有重要医学意义的黄病毒是黄热病、登革热、圣刘易斯脑炎、西尼罗河和罗西奥病毒。Rocio病毒（ROCV）是巴西严重脑炎流行病的原因，病死率为10%，20%的幸存者出现后遗症。我们首次对ROCV全基因组进行了测序和鉴定，确定了开放阅读框架的一般特征和病毒基因的特征，包括潜在的切割位点、保守或独特的基序、半胱氨酸残基和潜在的糖基化位点。鉴定了3 '-非编码区的保守序列，并研究了5'-和3 '-非编码区之间环化过程中预测的二级结构。基于抗原重要性和遗传信息基因的多蛋白和系统发育分析证实了ROCV和伊尔赫斯病毒（ILHV）之间的密切关系，共同构成了一个独特的和不同的系统发育亚组，以及ROCV与日本脑炎组的几个成员的遗传关系。虽然ROCV与ILHV在遗传学上相关，但我们的研究表明它仍然是一种不同于后者的病毒。这是巴西特有的第一种黄病毒，已被完全测序，基因组已被鉴定。这些数据将有助于进一步在分子水平上进行研究，包括构建感染性克隆、鉴定基因功能、改进基于分子诊断工具的疾病监测和疫苗开发。
The flaviviruses of major medical importance in South American countries are yellow fever, dengue, Saint Louis encephalitis, West Nile and Rocio viruses. Rocio virus (ROCV) has been responsible for epidemics of severe encephalitis in Brazil with a case-fatality rate of 10 % and development of sequelae in 20% of the survivors. We have sequenced and characterized the entire genome of ROCV for the first time, by determining the general traits of the open reading frame and the characteristics of viral genes including the potential cleavage sites, conserved or unique motifs, cysteine residues and potential glycosylation sites. The conserved sequences in the 3'-non-coding region were identified, and the predicted secondary structures during cyclization between 5'- and 3'-non-coding regions were studied. Multiple protein and phylogenetic analyses based on antigenically important and phylogenetically informative genes confirmed a close relationship between ROCV and Ilheus virus (ILHV), together constituting a unique and distinct phylogenetic subgroup as well as the genetic relationship of ROCV with several members of the Japanese encephalitis group. Although ROCV is phylogenetically related to ILHV, our study shows that it is still a virus distinct from the latter virus. This is the first flavivirus uniquely indigenous to Brazil that has been sequenced completely and the genome characterized. The data should be useful for further studies at the molecular level, including construction of infectious clone, identification of gene function, improved disease surveillance based on molecular diagnostic tools and vaccine development.