Exploring the key genes and pathways in enchondromas using a gene expression microarray.
Exploring the key genes and pathways in enchondromas using a gene expression microarray.
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使用基因表达微阵列探索内生软骨瘤的关键基因和途径
DOI：
10.18632/oncotarget.16700
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发表时间：
2017-07-04
期刊：
影响因子：
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通讯作者：
Feng S
中科院分区：
文献类型：
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作者：
Shi Z;Zhou H;Pan B;Lu L;Kang Y;Liu L;Wei Z;Feng S
内生软骨瘤是发生在髓腔内最常见的原发性良性骨肿瘤，它可发生恶变转化为软骨肉瘤。然而，内生软骨瘤在患者中常常未被检测到，其分子机制尚不清楚。为了确定与内生软骨瘤发生和发展相关的关键基因和通路，我们下载了基因表达数据集GSE22855，并通过分析内生软骨瘤中的高通量基因表达获得了差异表达基因（DEGs）。总共鉴定出635个基因作为差异表达基因。其中，225个基因（35.43%）上调，其余410个基因（64.57%）下调。我们确定了在内生软骨瘤样本中与对照样本相比显著过度表达的主要基因本体（GO）类别和京都基因与基因组百科全书（KEGG）通路。随后从蛋白质 - 蛋白质相互作用（PPI）网络中鉴定出前10个核心基因。对主要涉及两个重要模块的基因进行的富集分析表明，差异表达基因主要与核糖体、蛋白质消化和吸收、细胞外基质 - 受体相互作用、粘着斑、阿米巴病和PI3K - Akt信号通路有关。
总之，这些数据阐明了内生软骨瘤发生和发展的分子机制，并为治疗干预和预后评估提供了有希望的候选基因。然而，需要进一步的实验研究来证实这些结果。
Enchondromas are the most common primary benign osseous neoplasms that occur in the medullary bone; they can undergo malignant transformation into chondrosarcoma. However, enchondromas are always undetected in patients, and the molecular mechanism is unclear. To identify key genes and pathways associated with the occurrence and development of enchondromas, we downloaded the gene expression dataset GSE22855 and obtained the differentially expressed genes (DEGs) by analyzing high-throughput gene expression in enchondromas. In total, 635 genes were identified as DEGs. Of these, 225 genes (35.43%) were up-regulated, and the remaining 410 genes (64.57%) were down-regulated. We identified the predominant gene ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways that were significantly over-represented in the enchondromas samples compared with the control samples. Subsequently the top 10 core genes were identified from the protein-protein interaction (PPI) network. The enrichment analyses of the genes mainly involved in two significant modules showed that the DEGs were principally related to ribosomes, protein digestion and absorption, ECM-receptor interaction, focal adhesion, amoebiasis and the PI3K-Akt signaling pathway. Together, these data elucidate the molecular mechanisms underlying the occurrence and development of enchondromas and provide promising candidates for therapeutic intervention and prognostic evaluation. However, further experimental studies are needed to confirm these results.