An archaeal bi-species biofilm formed by Pyrococcus furiosus and Methanopyrus kandleri
An archaeal bi-species biofilm formed by Pyrococcus furiosus and Methanopyrus kandleri
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DOI：
10.1007/s00203-008-0371-9
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Wirth, Reinhard
中科院分区：
文献类型：
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作者：
Schopf, Simone;Wanner, Gerhard;Wirth, Reinhard
最近的研究表明，激烈火球菌不仅使用其鞭毛游泳，而且还用于建立细胞间的连接，并粘附到非生物表面。因此，这里有人问，P. furiosus是否也能够粘附到生物表面。由于Methanopyrus kandleri可以在类似于P. furiosus的栖息地（接近沸点和厌氧条件的海水）中发现，因此测试了两种古菌之间是否发生相互作用。使用标准介质和在H（2）中还原的气相（与M. Kandleri），我们能够在稳定的共培养物中培养两个物种。非常有趣，M。kandleri可以在这种条件下粘附到玻璃上，但P. furiosus不能。然而，后一种古菌能够附着在M. Kandleri细胞并附着在其自身上，从而在玻璃上形成结构化的生物膜。这些通常作为M的底层出现。kandleri细胞被大量的P. furiosus细胞覆盖。激烈拟杆菌与M. kandleri不仅通过鞭毛介导，而且通过直接的细胞-细胞接触介导。
Recently it was shown that Pyrococcus furiosus uses its flagella not only for swimming, but also for establishment of cell-cell connections, and for adhesion to abiotic surfaces. Therefore, it was asked here if P. furiosus might be able to adhere also to biotic surfaces. Since Methanopyrus kandleri can be found in habitats similar to those of P. furiosus (seawater close to the boiling point and anaerobic conditions) it was tested if interactions between both archaea occur. Using a standard medium and a gas phase reduced in H(2) (compared with the optimal gas phase for M. kandleri) we were able to grow both species in a stable coculture. Very interestingly, M. kandleri could adhere to glass under such conditions, but not P. furiosus. This latter archaeum, however, was able to adhere onto M. kandleri cells and onto itself, resulting in structured biofilms on glass. These very often appeared as a bottom layer of M. kandleri cells covered by a multitude of P. furiosus cells. Interactions between P. furiosus and M. kandleri were mediated not only by flagella, but also by direct cell-cell contact.