EUKARYOTIC DNA METHYLATION - FACTS AND PROBLEMS
EUKARYOTIC DNA METHYLATION - FACTS AND PROBLEMS
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DOI：
10.1016/0014-5793(90)81280-2
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发表时间：
1990-08-01
期刊：
影响因子：
3.5
通讯作者：
OREND, G
中科院分区：
文献类型：
--
作者：
DOERFLER, W;TOTH, M;OREND, G
在哺乳动物细胞等复杂基因组中，DNA甲基化模式被视为不同水平遗传活动的指标。目前尚不清楚这些复杂的模式是如何在细胞复制过程中产生和维持的。许多不同生物系统的证据表明，高等真核生物启动子的序列特异性甲基化是长期控制基因活性的重要因素之一。一般来说，第5个核苷酸5-甲基脱氧胞苷可以被认为是蛋白质- dna相互作用的调节剂。这种调制的程度和方向必须在每个单独的实例中进行实验评估。DNA甲基化的新模式的建立以DNA甲基化的逐渐非随机扩散为特征，其机制基本上是未知的。在这篇综述中，介绍了哺乳动物系统中DNA甲基化的一些一般概念，并对作者实验室目前进行的研究进行了总结。
Patterns of DNA methylation in complex genomes like those of mammalian cells have been viewed as indicators of different levels of genetic activities. It is as yet unknown how these complicated patterns are generated and maintained during cell replication. There is evidence from many different biological systems that the sequence-specific methylation of promoters in higher eukaryotes is one of the important factors in controlling gene activity at a long-term level. In general, the fifth nucleotide 5-methyldeoxycytidine can be considered as a modulator of protein-DNA interactions. The degree and direction of this modulation has to be assessed experimentally in each individual instance. The establishment of de novo patterns of DNA methylation is characterized by the gradual non-random spreading of DNA methylation by an essentially unknown mechanism. In this review, some of the general concepts of DNA methylation in mammalian systems are presented, and research currently performed in the authors' laboratory has been summarized.