Comprehensive analysis of VQ motif-containing gene expression in rice defense responses to three pathogens
Comprehensive analysis of VQ motif-containing gene expression in rice defense responses to three pathogens
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DOI：
10.1007/s00299-014-1633-4
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发表时间：
2014-09-01
影响因子：
6.2
通讯作者：
Wang, Shiping
中科院分区：
文献类型：
--
作者：
Li, Na;Li, Xianghua;Wang, Shiping
水稻VQ（FxxxVQxLTG）基序基因在病原菌侵染应答中的表达水平因病原菌而异，其中一些基因与防御应答基因WRKY共表达，最近的研究发现，植物中一些VQ（FxxxVQxLTG）基序蛋白与WRKY转录因子共同参与其功能。研究结果表明，WRKY蛋白在水稻对病原菌侵染的应答中起着重要作用。然而，水稻中含有VQ基序的蛋白质的功能尚不清楚。为了探讨水稻中含有VQ基序的蛋白质是否参与了对病原菌的防御，我们对这些蛋白质的基因进行了全面的表达分析。水稻含VQ基序家族由40个基因组成，所有基因编码的蛋白质含有21个氨基酸的含VQ基序，而该基序又含有已知的VQ基序。在系统发育关系、组织特异性表达和发育阶段特异性表达特征的基础上，对水稻对3种病原菌（白叶枯病菌（Xanthomonas oryzae pv.水稻白叶枯病的致病性; oryzaepv.稻瘟病菌，其引起细菌性条斑病;以及稻瘟病菌，其引起真菌性稻瘟病。一些基因的表达在应答至少一种病原体种属的感染时显著改变，并且一些基因在抗性和易感性反应中也显示出显著不同的表达。此外，一些含有防御应答VQ基序的基因与防御应答WRKY基因共表达。这些结果为研究水稻VQ基序蛋白及其WRKY伴侣在水稻与病原菌互作中的作用提供了新的视角。
Expression levels of rice VQ motif-containing genes in response to pathogen infection vary among pathogens, and some of the genes are co-expressed with defense-response WRKY genes.Recent studies have revealed that some VQ (FxxxVQxLTG) motif-containing proteins in plants partner with WRKY transcription factors to participate in their functions. Accumulating information suggests that WRKY proteins play important roles in the response of rice plants to pathogen infection. However, the functions of rice VQ motif-containing proteins are unknown. To explore whether VQ motif-containing proteins are involved in defense against pathogens in rice, we performed a comprehensive expression analysis of the genes for these proteins. The rice VQ motif-containing family consists of 40 genes, all of which encode proteins harboring a 21-amino acid VQ-containing motif, which in turn contains the known VQ motif. On the basis of their phylogenetic relationships and tissue-specific and developmental stage-specific expression characteristics, we transcriptionally analyzed 13 representative genes in rice responses to three pathogens: Xanthomonas oryzae pv. oryzae, which causes bacterial blight disease; X. oryzae pv. oryzicola, which causes bacterial streak disease; and Magnaporthe oryzae, which causes fungal blast disease. The expression of some of the genes changed markedly in response to infection by at least one of the pathogen species, and some of the genes also showed markedly different expression in resistant and susceptible reactions. In addition, some defense-responsive VQ motif-containing genes were co-expressed with defense-response WRKY genes. These results provide a new perspective on the putative roles of rice VQ motif-containing proteins and their putative WRKY partners in rice-pathogen interactions.