MALDI-Based Mass Spectrometry in Clinical Testing: Focus on Bacterial Identification
MALDI-Based Mass Spectrometry in Clinical Testing: Focus on Bacterial Identification
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DOI：
10.3390/app12062814
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发表时间：
2022-03-01
影响因子：
2.7
通讯作者：
Nakayama, Tomohiro
中科院分区：
文献类型：
--
作者：
Tsuchida, Sachio;Nakayama, Tomohiro
术语“蛋白质组”是指在生物体中表达的所有蛋白质的总和。术语“蛋白质组学”是指不仅包括关于单个蛋白质的表达水平的信息，而且包括它们的高级结构、分子间相互作用和翻译后修饰的研究领域。核心技术，基质辅助激光解吸/电离质谱法（MALDI-MS），可用于蛋白质分析，这要归功于2002年获得诺贝尔化学奖的Koichi Tanaka和John Fenn的工作。在临床实践中，最成功的蛋白质组分析是快速微生物鉴定。该方法通过将由基质辅助激光解吸电离飞行时间MS（MALDI-TOF MS）获得的细菌的蛋白质组谱与数据库进行比较来确定细菌种类。MS在简单性、速度和准确性方面上级经典的染色和表型物种形成。在临床微生物学中，MS对感染性疾病的诊断和治疗产生了很大的影响。感染性疾病的早期诊断和治疗非常重要，MALDI-TOF MS的快速鉴定为该领域做出了重大贡献。
The term "proteome" refers to the total of all proteins expressed in an organism. The term "proteomics" refers to the field of research that includes not only information on the expression levels of individual proteins, but also their higher-order structures, intermolecular interactions, and post-translational modifications. The core technology, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), is available for protein analysis thanks to the work of Koichi Tanaka and John Fenn, who were awarded the Nobel Prize in Chemistry in 2002. The most successful proteome analysis in clinical practice is rapid microbial identification. This method determines the bacterial species by comparing the proteome profile of the bacteria obtained by matrix-assisted laser desorption ionization-time of flight MS (MALDI-TOF MS) with a database. MS is superior in simplicity, speed, and accuracy to classic speciation by staining and phenotyping. In clinical microbiology, MS has had a large impact on the diagnosis and treatment of infectious disease. Early diagnosis and treatment of infectious disease are important, and rapid identification by MALDI-TOF MS has made a major contribution to this field.