Origin of endothelial progenitors in human postnatal bone marrow.
Origin of endothelial progenitors in human postnatal bone marrow.
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DOI：
10.1172/jci14327c1
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发表时间：
2002-02
期刊：
影响因子：
--
通讯作者：
Morayma Reyes;Arkadiusz Dudek;B. Jahagirdar;L. Koodie;P. Marker;Catherine M. Verfaillie
中科院分区：
文献类型：
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作者：
Morayma Reyes;Arkadiusz Dudek;B. Jahagirdar;L. Koodie;P. Marker;Catherine M. Verfaillie
这项研究表明，CD 34（-），血管内皮钙粘蛋白（-）（VE-钙粘蛋白（-）），AC 133（+），和胎肝激酶（+）（Flk 1（+））多能成人祖细胞（MAPC），与来自出生后的人骨髓（BM）的间充质干细胞共纯化，是成血管细胞的祖细胞。在体外，与VEGF一起培养的MAPCs分化为CD 34（+）、VE-钙粘蛋白（+）、Flk 1（+）细胞--预期为成血管细胞的表型。随后，它们分化为表达内皮标志物的细胞，在体外发挥成熟内皮细胞的功能，并在肿瘤血管生成和伤口愈合期间促进体内新血管生成。这种成血管细胞前体向内皮细胞分化的体外模型在研究成血管细胞及其他细胞的定向分化方面应该是非常有用的。在体内，MAPCs可以响应于局部信号而分化成有助于肿瘤中的新血管生成的内皮细胞。因为MAPCs可以在培养物中扩增而没有明显的衰老超过80个群体，所以它们可能是用于细胞促或抗血管生成疗法的内皮细胞的重要来源。
This study demonstrates that a CD34(-), vascular endothelial cadherin(-) (VE-cadherin(-)), AC133(+), and fetal liver kinase(+) (Flk1(+)) multipotent adult progenitor cell (MAPC) that copurifies with mesenchymal stem cells from postnatal human bone marrow (BM) is a progenitor for angioblasts. In vitro, MAPCs cultured with VEGF differentiate into CD34(+), VE-cadherin(+), Flk1(+) cells - a phenotype that would be expected for angioblasts. They subsequently differentiate into cells that express endothelial markers, function in vitro as mature endothelial cells, and contribute to neoangiogenesis in vivo during tumor angiogenesis and wound healing. This in vitro model of preangioblast-to-endothelium differentiation should prove very useful in studying commitment to the angioblast and beyond. In vivo, MAPCs can differentiate in response to local cues into endothelial cells that contribute to neoangiogenesis in tumors. Because MAPCs can be expanded in culture without obvious senescence for more than 80 population doublings, they may be an important source of endothelial cells for cellular pro- or anti-angiogenic therapies.