DEVELOPMENT OF MICROGLIAL TOPOGRAPHY IN HUMAN RETINA
DEVELOPMENT OF MICROGLIAL TOPOGRAPHY IN HUMAN RETINA
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DOI：
10.1002/cne.903630106
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发表时间：
1995-12-04
影响因子：
2.5
通讯作者：
BILLSON, FA
中科院分区：
文献类型：
--
作者：
DIAZARAYA, CM;PROVIS, JM;BILLSON, FA
在年龄范围内，使用组织化学和免疫组织化学的CD45和主要的组织相容性复合物II类抗原在10-25周妊娠（WG）中检查了人类视网膜全山脉中的小胶质形状的发展。小胶质细胞存在于与发育中的神经纤维层/神经节细胞层，内丛状层和外丛状层相对应的三个平面中。通过视网膜厚度和视网膜表面积的细胞分布模式随胎龄而变化。小胶质细胞在浅表视网膜中拉长，大型且在中间平面上被延伸，并且在深度视网膜中较小，圆形且不受影响。在睫状过程的caeca处看到的深刻标记，圆形的轮廓，视网膜边缘和视盘可能代表某些视网膜小胶质细胞的前体。在10 wg时，在远处和视网膜边缘的中部和深度视网膜中，小胶质细胞的密度最高，而在视盘则很少有浅表小胶质细胞。在14 wg时，高密度的小胶质细胞在视盘上表现出明显的表面。中央和深视网膜的小胶质细胞分布在更中心的位置，尽管继续集中在视网膜外围。小胶质细胞似乎从两个电源源和视盘迁移到发展中的人视频，最有可能源自纤毛体和虹膜的血管以及视网膜脉管系统。数据表明，小胶质细胞的发展发生在两个阶段，一个早期阶段发生在血管化之前，并且与视网膜血管的发展有关。 （c）1995 Wiley-Liss，Inc。
The development of microglial topography in wholemounts of human retina has been examined in the age range 10-25 weeks gestation (WG) using histochemistry and immunohistochemistry for CD45 and major histocompatibility complex class II antigens. Microglia were present in three planes corresponding to the developing nerve fibre layer/ganglion cell layer, the inner plexiform layer and the outer plexiform layer. Distribution patterns of cells through the retinal thickness and across the retinal surface area varied with gestational age. Microglia were elongated in superficial retina, large and ramified in the middle plane, and small, rounded and less ramified in deep retina. Intensely labeled, rounded profiles seen at the pars caeca of the ciliary processes, the retinal margin and at the optic disc may represent precursors of some retinal microglia. At 10 WG, the highest densities of microglia were present in middle and deep retina in the far periphery and at the retinal margin, with few superficial microglia evident centrally at the optic disc. At 14 WG, high densities of microglia were apparent superficially at the optic disc; microglia of middle and deep retina were distributed at more central locations although continuing to concentrate in the retinal periphery. Microglia appear to migrate into the developing human retina from two mains sources, the retinal margin and the optic disc, most likely originating from the blood vessels of the ciliary body and iris, and the retinal vasculature, respectively. The data suggest that the development of microglial topography occurs in two phases, an early phase occurring prior to vascularization, and a late phase associated with the development of the retinal vasculature. (C) 1995 Wiley-Liss, Inc.