Nanoindentation of histological specimens: Mapping the elastic properties of soft tissues.
Nanoindentation of histological specimens: Mapping the elastic properties of soft tissues.
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DOI：
10.1557/jmr.2009.0130
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发表时间：
2009-03
影响因子：
2.7
通讯作者：
Derby B
中科院分区：
文献类型：
--
作者：
Akhtar R;Schwarzer N;Sherratt MJ;Watson RE;Graham HK;Trafford AW;Mummery PM;Derby B
虽然动态和顺应性组织的总体机械特性的改变对人类健康和发病率具有重大影响，但还没有成熟的技术来表征组织（例如血管和肺）的微观机械特性。我们已经使用纳米压痕空间映射的5 μ m厚的部分雪貂主动脉和腔静脉的微观力学性能，并将这些力学性能的荧光弹性纤维的组织学分布。为了解耦玻璃基板对我们的纳米压痕数据的分析的影响，我们使用了扩展的奥利弗和法尔方法。主动脉的弹性模量从外膜（最外层）的35 MPa逐渐降低到内膜（最内层）的8 MPa。相比之下，腔静脉相对较硬，血管富含细胞外基质的外膜和内膜区域的弹性模量均>30 MPa。然而，腔静脉的中央、高度细胞化的中间层具有约20 MPa的不变弹性模量。在细胞外基质丰富的区域的组织，弹性模量，确定纳米压痕，与弹性纤维密度呈负相关。因此，我们表明，有可能区分和空间解决大动脉和静脉，这是相关的组织微观结构的微观力学性能的差异。
Although alterations in the gross mechanical properties of dynamic and compliant tissues have a major impact on human health and morbidity, there are no well-established techniques to characterize the micromechanical properties of tissues such as blood vessels and lungs. We have used nanoindentation to spatially map the micromechanical properties of 5-μm-thick sections of ferret aorta and vena cava and to relate these mechanical properties to the histological distribution of fluorescent elastic fibers. To decouple the effect of the glass substrate on our analysis of the nanoindentation data, we have used the extended Oliver and Pharr method. The elastic modulus of the aorta decreased progressively from 35 MPa in the adventitial (outermost) layer to 8 MPa at the intimal (innermost) layer. In contrast, the vena cava was relatively stiff, with an elastic modulus >30 MPa in both the extracellular matrix-rich adventitial and intimal regions of the vessel. The central, highly cellularized, medial layer of the vena cava, however, had an invariant elastic modulus of ~20 MPa. In extracellular matrix-rich regions of the tissue, the elastic modulus, as determined by nanoindentation, was inversely correlated with elastic fiber density. Thus, we show it is possible to distinguish and spatially resolve differences in the micromechanical properties of large arteries and veins, which are related to the tissue microstructure.