Hair follicle reformation induced by dermal papilla cells from human scalp skin
Hair follicle reformation induced by dermal papilla cells from human scalp skin
复制标题
人头皮真皮乳头细胞诱导毛囊重建
DOI：
10.1007/s00403-006-0686-9
复制
发表时间：
2006-09-01
影响因子：
3
通讯作者：
Tang, Shu-Qian
中科院分区：
文献类型：
--
作者：
Wu, Jin-Jin;Zhu, Tang-You;Tang, Shu-Qian
探讨毛乳头细胞体内外诱导毛囊再生的可能性。将酶消化法获得的人头皮真皮乳头细胞、真皮鞘细胞与胶原蛋白混合，形成间充质细胞填充的胶原凝胶。将上、下层上皮细胞和球部基质细胞通过器官型培养在凝胶上进行培养，重组双层人工皮肤。将毛乳头细胞和外根鞘角质形成细胞混合后移植到裸鼠背部皮下。HE染色观察组织学检查结果。通过器官型培养，这些重组子都形成了类似天然皮肤的双层结构。真皮鞘细胞填充的胶原凝胶与上、下层上皮细胞结合后可见毛囊样结构重塑。毛乳头细胞还可诱导上、下层上皮细胞在裸鼠体内形成毛囊。低传代毛乳头细胞与毛囊上皮细胞混合移植到裸鼠体内后，可重塑许多典型的毛囊结构，形成毛发纤维。毛囊的真皮部分，如毛乳头细胞和真皮鞘细胞，通过与毛囊上皮细胞相互作用，具有诱导毛囊形成的能力。
To investigate the possibility of hair follicle reformation induced by dermal papilla cells in vivo and in vitro. Dermal papilla cells, dermal sheath cells obtained from human scalp skin by enzyme digestion were mixed with collagen to form mesenchymal cell-populated collagen gels. Superior and inferior epithelial cells and bulb matrical cells were then cultured on these gels by organotypic culture to recombine bilayer artificial skins. Dermal papilla cells and outer root sheath keratinocytes were mingled together and transplanted under subcutaneous tissue of the dorsal skin of nude mice. The results of histologic examination was observed with HE stain. These recombinants by organotypic culture all reformed bilayer structure like nature skin. Hair follicle-like structure reformation was found in dermal sheath cell-populated collagen gel when combined with superior or inferior epithelial cells. Dermal papilla cells also induced superior and inferior epithelial cells to form hair follicle on nude mice. Low passage dermal papilla cells mixed with hair follicle epithelial cells reformed many typical hair follicle structures and produced hair fibres after transplantation on nude mice. The dermal part of hair follicle, such as dermal papilla cells and dermal sheath cells, has the ability to induce hair follicle formation by interaction with the epithelial cells of hair follicle.