Gene promoters dictate histone occupancy within genes.
Gene promoters dictate histone occupancy within genes.
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基因启动子决定基因内组蛋白的占据。
DOI：
10.1038/emboj.2013.194
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Bentley,David
中科院分区：
文献类型：
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作者：
Perales,Roberto;Erickson,Benjamin;Zhang,Lian;Kim,Hyunmin;Valiquett,Elan;Bentley,David
Spt6 is a transcriptional elongation factor and histone chaperone that reassembles transcribed chromatin. Genome‐wide H3 mapping showed that Spt6 preferentially maintains nucleosomes within the first 500 bases of genes and helps define nucleosome‐depleted regions in 5′ and 3′ flanking sequences. In Spt6‐depleted cells, H3 loss at 5′ ends correlates with reduced pol II density suggesting enhanced transcription elongation. Consistent with its ‘Suppressor of Ty’ (Spt) phenotype, Spt6 inactivation caused localized H3 eviction over 1–2 nucleosomes at 5′ ends of Ty elements. H3 displacement differed between genes driven by promoters with ‘open’/DPN and ‘closed’/OPN chromatin conformations with similar pol II densities. More eviction occurred on genes with ‘closed’ promoters, associated with ‘noisy’ transcription. Moreover, swapping of ‘open’ and ‘closed’ promoters showed that they can specify distinct downstream patterns of histone eviction/deposition. These observations suggest a novel function for promoters in dictating histone dynamics within genes possibly through effects on transcriptional bursting or elongation rate.
