end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos.
end-1 encodes an apparent GATA factor that specifies the endoderm precursor in Caenorhabditis elegans embryos.
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DOI：
10.1101/gad.11.21.2883
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发表时间：
1997-11
影响因子：
10.5
通讯作者：
Jiangwen Zhu;R. J. Hill;P. Heid;Masamitsu Fukuyama;A. Sugimoto;J. Priess;J. Rothman
中科院分区：
文献类型：
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作者：
Jiangwen Zhu;R. J. Hill;P. Heid;Masamitsu Fukuyama;A. Sugimoto;J. Priess;J. Rothman
线虫秀丽隐杆线虫的内胚层是从 E 创始细胞克隆衍生的。我们确定了产生整个秀丽隐杆线虫内胚层所需的单个基因组区域（内胚层决定区域或 EDR）。在缺乏 EDR 的胚胎中，E 细胞产生外胚层和中胚层而不是内胚层，并且似乎继承了其表亲 C 创始细胞的命运。 end-1 是 EDR 的一个基因，可恢复 EDR 缺陷纯合子的内胚层生成。 end-1 转录物首先在 E 细胞中特异性检测到，这与 end-1 在内胚层发育中的直接作用一致。 END-1 蛋白是一种明显的含锌指 GATA 转录因子。由于 GATA 因子与其他动物的内胚层发育有关，我们的研究结果表明内胚层可能是由三叶细胞动物中的分子保守机制指定的。我们认为，end-1是已知参与线虫胚层和创始细胞身份规范的第一个合子基因，它可能将调节内胚层建立的母体基因与负责内胚层分化的下游基因联系起来。
The endoderm in the nematode Caenorhabditis elegans is clonally derived from the E founder cell. We identified a single genomic region (the endoderm-determining region, or EDR) that is required for the production of the entire C. elegans endoderm. In embryos lacking the EDR, the E cell gives rise to ectoderm and mesoderm instead of endoderm and appears to adopt the fate of its cousin, the C founder cell. end-1, a gene from the EDR, restores endoderm production in EDR deficiency homozygotes. end-1 transcripts are first detectable specifically in the E cell, consistent with a direct role for end-1 in endoderm development. The END-1 protein is an apparent zinc finger-containing GATA transcription factor. As GATA factors have been implicated in endoderm development in other animals, our findings suggest that endoderm may be specified by molecularly conserved mechanisms in triploblastic animals. We propose that end-1, the first zygotic gene known to be involved in the specification of germ layer and founder cell identity in C. elegans, may link maternal genes that regulate the establishment of the endoderm to downstream genes responsible for endoderm differentiation.