Up-regulated death-associated LIM-only protein contributes to fitness costs of Bacillus thuringiensis Cry1Ac resistance in Helicoverpa armigera.
Up-regulated death-associated LIM-only protein contributes to fitness costs of Bacillus thuringiensis Cry1Ac resistance in Helicoverpa armigera.
复制标题
DOI：
10.1016/j.jinsphys.2013.11.011
复制
发表时间：
2014
影响因子：
2.2
通讯作者：
Huiming Guo;Guo-qing Lu;Xiaofeng Su;G. Liang;Chenxi Liu;Hongmei Cheng
中科院分区：
文献类型：
--
作者：
Huiming Guo;Guo-qing Lu;Xiaofeng Su;G. Liang;Chenxi Liu;Hongmei Cheng
苏云金芽孢杆菌(Bt)抗性的进化为研究与胁迫适应相关的适应度权衡提供了一条有用的途径。在以前的研究中，我们利用cDNA扩增片段长度多态(cDNA-ALFP)分析来鉴定棉铃虫对Cry1Ac敏感(96S)和抗性(Bt-R)的差异表达基因(Deg)。在这些DEG中，鉴定出一种死亡相关LIM-Only蛋白(HaDALP)。在本研究中，根据先前的cDNA-ALFP分析得到的表达序列标签，采用快速扩增的方法克隆了编码HaDALP的全长cDNA。HaDALP的表达谱表明，该基因在组织和发育阶段均有差异表达，在5龄幼虫的中肠和表皮中表达水平最高。它在抗Cry1Ac的H中上调。棉铃虫。在体内敲除或摄食HaDALP蛋白后，评估了最敏感的指标，如幼虫和蛹的重量、蛹的持续时间和存活率。我们的研究结果表明，HaDALP的上调可能与对Cry1Ac的抗性和对Bt毒素的适应有关。
The evolution ofBacillus thuringiensis(Bt) resistance provides a useful pathway for the study of fitness trade-offs associated with stress adaptation. In a previous study, we used cDNA-amplification fragment length polymorphism (cDNA-ALFP) analysis to identify differentially expressed genes (DEGs) in Cry1Ac-susceptible (96S) and -resistant (Bt-R)Helicoverpa armigeralarvae. Among these DEGs, a death-associated LIM-only protein (HaDALP) was identified. In the current study, the full-length cDNA encoding HaDALP was cloned using rapid amplification of cDNA ends according to an expressed sequence tag derived from the previous cDNA-ALFP analysis.HaDALPexpression patterns indicated that this gene was differentially expressed in tissues and stages and was highest in the midgut and epidermis of the 5th instar larvae. It is up-regulated in Cry1Ac-resistantH. armigeralarvae. Fitness parameters, such as larval and pupal weight, pupal duration, and survival rate, which are the most sensitive indicators, were evaluated afterHaDALPknockdown or feeding of the HaDALP proteinin vivo. Our findings suggested that up-regulation of HaDALP might be related to Cry1Ac resistance and an adaption to Bt toxins.