Automatic analysis for amperometrical recordings of exocytosis
Automatic analysis for amperometrical recordings of exocytosis
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DOI：
10.1016/s0165-0270(00)00309-5
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发表时间：
2000-11-30
影响因子：
3
通讯作者：
Borges, R
中科院分区：
文献类型：
--
作者：
Segura, F;Brioso, MA;Borges, R
电流法是一种广泛使用的技术，用于监测分泌的儿茶酚胺（CA）的胞吐。碳纤维微电极的使用允许从单个分泌囊泡释放的CA的在线记录。电流信号是由靠近电极头端的定量释放的CA氧化产生的。每一个胞吐事件被称为分泌峰。本文介绍了一个为IGOR（Wavemetrics，Lake奥斯韦戈，OR，USA）编写的程序，它可用于电流信号的离线分析。该程序允许：（i）数字滤波和分析电流噪声，尖峰识别和尖峰动力学参数的计算;（ii）尖峰审查;（iii）汇集尖峰和数据，以创建测量参数的图库、表格和直方图，这些参数可以导出为图形格式或文件，以供进一步分析。(C)2000 Elsevier Science B. V.保留所有权利。
Amperometry is a widely used technique for monitoring the secretion of catecholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allows the on-line recording of CA released from a single secretory vesicle. Amperometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Oswego, OR, USA), which may be used to analyze amperometric signals off-line. The procedures allow, (i) digital filtering and analysis of the current noise, spike identification and calculation of spike kinetic parameters: (ii) spike review; (iii) pooling spikes and data to create galleries, tables and histograms of measured parameters which can be exported to a graphic format or files for further analysis. (C) 2000 Elsevier Science B.V. All rights reserved.