Effect of forskolin on the expression of claudin-5 in human trophoblast BeWo cells
Effect of forskolin on the expression of claudin-5 in human trophoblast BeWo cells
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DOI：
10.1691/ph.2007.4.6679
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发表时间：
2007-04-01
期刊：
影响因子：
1.6
通讯作者：
Watanabe, Y.
中科院分区：
文献类型：
--
作者：
Harada, M.;Kondoh, M.;Watanabe, Y.
滋养层细胞是胎盘中发现的一种细胞类型，在胎盘作为母体液体和胎儿之间的屏障的功能中发挥着关键作用。最近，紧密蛋白（一种 24 kDa 的跨膜蛋白）被确定负责上皮细胞的屏障功能。在本研究中，我们研究了密蛋白的表达谱以及BeWo人滋养层细胞分化过程中表达的变化。逆转录聚合酶链反应和免疫印迹证明 BeWo 细胞中 Claudin-1、-3、-4 和 -5 的表达。 Forskolin 可诱导 BeWo 细胞从细胞滋养层样细胞分化为合体滋养层样细胞，并略微降低 Claudin-5 的表达。这是第一份显示经毛喉素处理的 BeWo 细胞中claudin-5 变化的报告。
Trophoblasts, a cell type found in the placenta, play a pivotal role in the function of the placenta as a barrier between the maternal fluid and the fetus. Recently, claudin, a 24-kDa transmembrane protein, was identified as being responsible for the barrier function of epithelia. In the present study, we investigated the expression profiles of claudin and the changes in expression during the differentiation of BeWo human trophoblast cells. Reverse transcriptase-polymerase chain reaction and immunoblotting demonstrated the expression of claudin-1, -3, -4, and -5 in BeWo cells. Forskolin, which induces the differentiation of BeWo cells from cytotrophoblast-like cells into syncytiotrophoblast-like cells, reduced slightly the expression of claudin-5. This is the first report to show changes in claudin-5 in forskolin-treated BeWo cells.