LAMININ SELECTIVELY ENHANCES AXONAL GROWTH AND ACCELERATES THE DEVELOPMENT OF POLARITY BY HIPPOCAMPAL-NEURONS IN CULTURE
LAMININ SELECTIVELY ENHANCES AXONAL GROWTH AND ACCELERATES THE DEVELOPMENT OF POLARITY BY HIPPOCAMPAL-NEURONS IN CULTURE
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DOI：
10.1016/0165-3806(92)90159-t
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发表时间：
1992-10-23
期刊：
影响因子：
--
通讯作者：
HIGGINS, D
中科院分区：
文献类型：
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作者：
LEIN, PJ;BANKER, GA;HIGGINS, D
我们研究了层粘连蛋白对组织培养的胚胎大鼠海马神经元形态发育的影响。接种后48小时，在聚赖氨酸包被的基质上生长的神经元已变得极化，通常具有一个长轴突和4或5个小突起。层粘连蛋白吸附到基板上并没有引起海马神经元延伸的轴突数量的变化，但确实引起了轴突丛长度和轴突分支的显着增加。与其对轴突的影响相反，层粘连蛋白并不影响最终成为树突的小突起的数量、长度或分支，也不影响培养7天后评估的明确树突的形态。除了选择性地促进轴突生长，层粘连蛋白大大增加了海马神经元的极化率，使得大多数在18小时内极化。层粘连蛋白的影响的时间过程的分析表明，极化的加速与初始过程形成的时间的变化无关，而是与在体外从第12小时到第48小时的所有时间的最长过程的生长的选择性刺激有关。这些数据表明，即使海马神经元的基本形状可能是内在编程，其形态发育的关键方面可能是由细胞外基质分子，如层粘连蛋白调制。
We have examined the effects of laminin on the morphological development of embryonic rat hippocampal neurons maintained in tissue culture. Forty-eight hours after plating, neurons grown on a polylysine-coated substrate had become polarized, typically having one long axon and 4 or 5 minor processes. Adsorption of laminin to the substrate did not cause changes in the number of axons extended by hippocampal neurons but did cause significant increases in the length of the axonal plexus and in axonal branching. In contrast to its effects on axons, laminin did not influence the number, length, or branching of the minor processes that eventually become dendrites or the morphology of definite dendrites as assessed after 7 days in culture. In addition to selectively enhancing axonal growth, laminin greatly increased the rate of polarization of hippocampal neurons such that most became polarized within 18 h. Analysis of the time course of laminin's effects revealed that the acceleration of polarization was not associated with a change in the time of initial process formation, but rather with a selective stimulation of the growth of the longest process at all times from the 12th through the 48th h in vitro. These data suggest that even though the basic shape of hippocampal neurons may be intrinsically programmed, critical aspects of their morphological development may be modulated by extracellular matrix molecules such as laminin.