KINECTIN, AN ESSENTIAL ANCHOR FOR KINESIN-DRIVEN VESICLE MOTILITY
KINECTIN, AN ESSENTIAL ANCHOR FOR KINESIN-DRIVEN VESICLE MOTILITY
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DOI：
10.1126/science.7892610
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发表时间：
1995-03-24
期刊：
影响因子：
56.9
通讯作者：
SHEETZ, MP
中科院分区：
文献类型：
--
作者：
KUMAR, J;YU, H;SHEETZ, MP
分子运动驱动蛋白的膜锚是参与细胞内膜运输的关键位点。特异性的单克隆抗体，细胞质表面的鸡脑微粒体被用来定义参与微管依赖性运输的蛋白质。测试的四种抗体中的一种即使作为单价Fab片段也抑制了约90%的正末端定向囊泡运动性，并降低了驱动蛋白与囊泡的结合。该抗体结合到kinectin的胞质结构域，kinectin是结合到驱动蛋白的内质网的整合膜蛋白。因此，kinectin作为一个膜锚蛋白驱动的囊泡运动。
The membrane anchor for the molecular motor kinesin is a critical site involved in intracellular membrane trafficking. Monoclonal antibodies specific for the; cytoplasmic surface of chick brain microsomes were used to define proteins involved in microtubule-dependent transport. One of four antibodies tested inhibited plus-end-directed vesicle motility by approximately 90 percent even as a monovalent Fab fragment and reduced kinesin binding to vesicles. This antibody bound to the cytoplasmic domain of kinectin, an integral membrane protein of the endoplasmic reticulum that binds to kinesin. Thus, kinectin acted as a membrane anchor protein for kinesin-driven vesicle motility.